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Virginijus Siksnys

Virginijus Siksnys

D-Index & Metrics

Molecular Biology

D-Index
50
Citations
16991
World Ranking
2530
National Ranking
1

Research.com Recognitions

  • 2019 - Member of Academia Europaea

Overview

Virginijus Siksnys is affiliated with Vilnius University in Lithuania and specializes in the field of Biochemistry, Genetics and Molecular Biology, with a primary focus on Molecular Biology. Their research extensively covers topics related to CRISPR and Genetic Engineering, RNA and protein synthesis mechanisms, and advanced biosensing and bioanalysis techniques. Other research interests include RNA regulation and disease, RNA modifications and cancer, cytomegalovirus and herpesvirus research, and bacteriophages and microbial interactions.

Recent publications by Virginijus Siksnys demonstrate contributions to understanding CRISPR systems and molecular mechanisms. Notable papers include:

  • PAM recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage, 2020, Nucleic Acids Research
  • Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease, 2021, Nature
  • A catalogue of biochemically diverse CRISPR-Cas9 orthologs, 2020, Nature Communications
  • Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion, 2022, Nature Microbiology
  • Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor, 2021, Nature Biomedical Engineering

Virginijus Siksnys frequently publishes research in several scientific journals and platforms, such as:

  • bioRxiv (Cold Spring Harbor Laboratory)
  • Nucleic Acids Research
  • Nature Communications
  • Biophysical Journal
  • Nature

The scientist collaborates frequently with a group of coauthors who have contributed significantly across multiple publications. These include:

  • Tautvydas Karvelis
  • Ralf Seidel
  • Česlovas Venclovas
  • Arūnas Šilanskas
  • Tomas Šinkūnas

Virginijus Siksnys has been recognized as a Member of Academia Europaea since 2019. Their work intersects multiple subfields of biological sciences, including Epidemiology, Genetics, Ecology, and Plant Science, with varying publication outputs.

Best Publications

  • Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

    Giedrius Gasiunas;Rodolphe Barrangou;Philippe Horvath;Virginijus Siksnys

  • Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants.

    Kira S. Makarova;Yuri I. Wolf;Jaime Iranzo;Sergey A. Shmakov

  • The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

    Rimantas Sapranauskas;Giedrius Gasiunas;Christophe Fremaux;Rodolphe Barrangou

  • Cas3 is a single‐stranded DNA nuclease and ATP‐dependent helicase in the CRISPR/Cas immune system

    Tomas Sinkunas;Giedrius Gasiunas;Christophe Fremaux;Rodolphe Barrangou

  • Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

    Mark D. Szczelkun;Maria S. Tikhomirova;Tomas Sinkunas;Giedrius Gasiunas

  • A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

    Migle Kazlauskiene;Georgij Kostiuk;Česlovas Venclovas;Gintautas Tamulaitis

  • Programmable rna shredding by the type iii-a crispr-cas system of streptococcus thermophilus

    Virginijus Šikšnys;Miglė Kazlauskienė;Gintautas Tamulaitis

  • crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus

    Tautvydas Karvelis;Giedrius Gasiunas;Algirdas Miksys;Rodolphe Barrangou

  • In vitro reconstitution of Cascade‐mediated CRISPR immunity in Streptococcus thermophilus

    Tomas Sinkunas;Giedrius Gasiunas;Sakharam P Waghmare;Mark J Dickman

  • Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome.

    Maximilian Müller;Ciaran M Lee;Ciaran M Lee;Giedrius Gasiunas;Timothy H Davis;Timothy H Davis

  • Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition

    Migle Kazlauskiene;Gintautas Tamulaitis;Georgij Kostiuk;Česlovas Venclovas

  • PAM recognition by miniature CRISPR-Cas12f nucleases triggers programmable double-stranded DNA target cleavage.

    Tautvydas Karvelis;Greta Bigelyte;Joshua K Young;Zhenglin Hou

  • RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX

    Virginijus Siksnys;Giedrius Gasiunas;Tautvydas Karvelis;Arvydas Lubys

  • Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

    Tautvydas Karvelis;Giedrius Gasiunas;Joshua Young;Greta Bigelyte

  • Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease.

    Tautvydas Karvelis;Gytis Druteika;Greta Bigelyte;Karolina Budre

  • Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA.

    Markus Deibert;Saulius Grazulis;Giedrius Sasnauskas;Virginijus Siksnys

  • Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection

    Marius Rutkauskas;Tomas Sinkunas;Inga Songailiene;Maria S. Tikhomirova

  • Novel subtype of type IIs restriction enzymes. BfiI endonuclease exhibits similarities to the EDTA-resistant nuclease Nuc of Salmonella typhimurium.

    Rimantas Sapranauskas;Giedrius Sasnauskas;Arunas Lagunavicius;Giedrius Vilkaitis

  • A catalogue of biochemically diverse CRISPR-Cas9 orthologs.

    Giedrius Gasiunas;Joshua K. Young;Tautvydas Karvelis;Darius Kazlauskas

  • Molecular mechanisms of CRISPR-mediated microbial immunity

    Giedrius Gasiunas;Tomas Sinkunas;Virginijus Siksnys

Frequent Co-Authors

Claus Urbanke
Claus Urbanke Hanover University of Applied Sciences and Arts
Robert Huber
Robert Huber University of Duisburg-Essen
Rodolphe Barrangou
Rodolphe Barrangou North Carolina State University
Stephen E. Halford
Stephen E. Halford University of Bristol
Yuri L. Lyubchenko
Yuri L. Lyubchenko University of Nebraska Medical Center
Robert Huber
Robert Huber Agricultural & Applied Economics Association
Eugene V. Koonin
Eugene V. Koonin National Institutes of Health
Michael Brudno
Michael Brudno University of Toronto
Kira S. Makarova
Kira S. Makarova National Institutes of Health
Yuri I. Wolf
Yuri I. Wolf National Institutes of Health

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