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Molecular Biology

D-Index
46
Citations
24550
World Ranking
2767
National Ranking
1332

Overview

Shengdar Q. Tsai is affiliated with St. Jude Children's Research Hospital in the United States and specializes mainly in biochemistry, genetics, and molecular biology. Their work spans multiple fields within medicine, with significant contributions to molecular biology, genetics, oncology, immunology, and pediatrics.

The research of Shengdar Q. Tsai largely focuses on genetic engineering and gene-editing technologies, particularly employing CRISPR methodologies. The main topics covered in their publications include:

  • CRISPR and Genetic Engineering
  • CAR-T cell therapy research
  • Hemoglobinopathies and Related Disorders
  • Virus-based gene therapy research
  • Prenatal Screening and Diagnostics
  • Immune Cell Function and Interaction
  • RNA modifications and cancer

Tsai has collaborated frequently with several co-authors over multiple publications. Notable frequent collaborators include Cícera R. Lazzarotto, Varun Katta, Yichao Li, Mitchell J. Weiss, and Jonathan Yen.

Their research outputs have appeared in a variety of scientific journals and preprint servers. Frequent publication venues include:

  • bioRxiv (Cold Spring Harbor Laboratory)
  • Blood
  • Nature
  • Nature Genetics
  • Nature Biomedical Engineering

Among the recent papers authored or co-authored by Tsai are:

  • Base editing of haematopoietic stem cells rescues sickle cell disease in mice, 2021, Nature
  • Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity, 2021, Science Translational Medicine
  • CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity, 2020, Nature Biotechnology
  • The NIH Somatic Cell Genome Editing program, 2021, Nature
  • Human genetic diversity alters off-target outcomes of therapeutic gene editing, 2022, Nature Genetics

Best Publications

  • Efficient genome editing in zebrafish using a CRISPR-Cas system

    Woong Y. Hwang;Yanfang Fu;Deepak Reyon;Morgan L. Maeder

  • High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

    Benjamin P. Kleinstiver;Vikram Pattanayak;Michelle S. Prew;Shengdar Q. Tsai

  • GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

    Shengdar Q Tsai;Zongli Zheng;Nhu T Nguyen;Matthew Liebers

  • Engineered CRISPR-CAS9 nucleases with altered PAM specificity

    J. Keith Joung;Benjamin Kleinstiver

  • FLASH assembly of TALENs for high-throughput genome editing

    Deepak Reyon;Shengdar Q Tsai;Cyd Khayter;Jennifer A Foden

  • Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

    Shengdar Q Tsai;Nicolas Wyvekens;Cyd Khayter;Jennifer A Foden

  • CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets

    Shengdar Q Tsai;Nhu T Nguyen;Jose Malagon-Lopez;Ved V Topkar

  • Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

    Benjamin P Kleinstiver;Shengdar Q Tsai;Michelle S Prew;Nhu T Nguyen

  • Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

    Benjamin P Kleinstiver;Michelle S Prew;Shengdar Q Tsai;Nhu T Nguyen

  • Toddler: an embryonic signal that promotes cell movement via Apelin receptors.

    Andrea Pauli;Megan L. Norris;Eivind Valen;Guo-Liang Chew

  • Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins

    Morgan L Maeder;James F Angstman;Marcy E Richardson;Samantha J Linder

  • Targeted disruption of DNMT1 , DNMT3A and DNMT3B in human embryonic stem cells

    Jing Liao;Rahul Karnik;Hongcang Gu;Michael J Ziller

  • Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases

    Shengdar Q. Tsai;J. Keith Joung

  • Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia

    Miriam Y. Kim;Kyung Rok Yu;Saad S. Kenderian;Marco Ruella

  • Highly efficient therapeutic gene editing of human hematopoietic stem cells.

    Yuxuan Wu;Jing Zeng;Jing Zeng;Benjamin P. Roscoe;Pengpeng Liu

  • High levels of AAV vector integration into CRISPR-induced DNA breaks

    Killian S. Hanlon;Benjamin P. Kleinstiver;Sara P. Garcia;Mikołaj P. Zaborowski;Mikołaj P. Zaborowski

  • In vivo CRISPR editing with no detectable genome-wide off-target mutations

    Pinar Akcakaya;Maggie L. Bobbin;Jimmy A. Guo;Jose Malagon-Lopez

  • Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs

    Lindsay Cade;Deepak Reyon;Woong Y. Hwang;Shengdar Q. Tsai

  • Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity

    John P Guilinger;Vikram Pattanayak;Deepak Reyon;Shengdar Q Tsai

  • 731. High-Fidelity CRISPR-Cas9 Nucleases with No Detectable Genome-Wide Off-Target Effects

    Benjamin P. Kleinstiver;Vikram Pattanayak;Michelle S. Prew;Shengdar Q. Tsai

Frequent Co-Authors

J. Keith Joung
J. Keith Joung Harvard University
David R. Liu
David R. Liu Broad Institute
Martin J. Aryee
Martin J. Aryee Harvard University
John F. Tisdale
John F. Tisdale National Institutes of Health
Daniel E. Bauer
Daniel E. Bauer Harvard University
Scot A. Wolfe
Scot A. Wolfe University of Massachusetts Chan Medical School
Mitchell J. Weiss
Mitchell J. Weiss St. Jude Children's Research Hospital
Alexander F. Schier
Alexander F. Schier University of Basel
Robert E. Donahue
Robert E. Donahue National Institutes of Health
John P. Manis
John P. Manis Boston Children's Hospital

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