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Molecular Biology

D-Index
47
Citations
8079
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2748
National Ranking
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Overview

Stephen Douthwaite is affiliated with the University of Southern Denmark in Denmark and focuses their research primarily in the field of Biochemistry, Genetics and Molecular Biology. Their work spans several subfields including Molecular Biology, Ecology, Parasitology, Food Science, and Epidemiology.

The main topics that characterize their research include:

  • RNA and protein synthesis mechanisms
  • Bacteriophages and microbial interactions
  • RNA modifications and cancer
  • Genomics and Phylogenetic Studies
  • Parasitic Infections and Diagnostics
  • Salmonella and Campylobacter epidemiology
  • Infective Endocarditis Diagnosis and Management

Douthwaite has authored several research papers in notable publication venues. Recent works include:

  • "Campylobacter jejuni Virulence Factors Identified by Modulating Their Synthesis on Ribosomes With Altered rRNA Methylation," 2022, Frontiers in Cellular and Infection Microbiology
  • "The hyperthermophilic partners Nanoarchaeum and Ignicoccus stabilize their tRNA T-loops via different but structurally equivalent modifications," 2020, Nucleic Acids Research
  • "Reductive Evolution and Diversification of C5-Uracil Methylation in the Nucleic Acids of Mollicutes," 2020, Biomolecules
  • "Virulence properties of Campylobacter jejuni are enhanced by displaying a mycobacterial TlyA methylation pattern in its rRNA," 2020, Cellular Microbiology
  • "Exploring a unique class of flavoenzymes: Identification and biochemical characterization of ribosomal RNA dihydrouridine synthase," 2024, Proceedings of the National Academy of Sciences

The frequent publication venues for Douthwaite's work include:

  • Frontiers in Cellular and Infection Microbiology
  • Proceedings of the National Academy of Sciences
  • Nucleic Acids Research
  • Biomolecules
  • Cellular Microbiology

Collaboration plays a significant role in their research activities. Frequent coauthors include:

  • Agnieszka Sałamaszyńska-Guz
  • Simon Rose
  • Małgorzata Murawska
  • Catherine Goyenvalle
  • Valérie de Crécy-Lagard

Best Publications

  • Basis for prokaryotic specificity of action of aminoglycoside antibiotics.

    Michael I. Recht;Stephen Douthwaite;Joseph D. Puglisi

  • Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action

    Liqun Xiong;Patricia Kloss;Stephen Douthwaite;Niels Møller Andersen

  • A "bulged" double helix in a RNA-protein contact site

    Debra A. Peattie;Steve Douthwaite;Roger A. Garrett;Harry F. Noller

  • Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 s ribosomal RNA from Escherichia coli

    Jan Egebjerg;Stephen R. Douthwaite;Anders Liljas;Roger A. Garrett

  • YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407.

    Niels Møller Andersen;Stephen Douthwaite

  • Interconversion of active and inactive 30 S ribosomal subunits is accompanied by a conformational change in the decoding region of 16 S rRNA

    Danesh Moazed;Barbara J. Van Stolk;Stephen Douthwaite;Harry F. Noller

  • Effect of mutations in the A site of 16 S rRNA on aminoglycoside antibiotic-ribosome interaction

    Michael I. Recht;Stephen Douthwaite;Kam D. Dahlquist;Joseph D. Puglisi

  • Resistance to the macrolide antibiotic tylosin is conferred by single methylations at 23S rRNA nucleotides G748 and A2058 acting in synergy

    Mingfu Liu;Stephen Douthwaite

  • Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK

    Laura Hidalgo;Katie L Hopkins;Belen Gutierrez;Cristina M Ovejero

  • Identifying modifications in RNA by MALDI mass spectrometry.

    Stephen Douthwaite;Finn Kirpekar

  • The structural basis of macrolide-ribosome binding assessed using mutagenesis of 23S rRNA positions 2058 and 2059

    Peter Pfister;Simon Jenni;Jacob Poehlsgaard;Ashley Thomas

  • Identifying the methyltransferases for m5U747 and m5U1939 in 23S rRNA using MALDI mass spectrometry

    Christian Toft Madsen;Jonas Mengel‐Jørgensen;Finn Kirpekar;Stephen Douthwaite

  • Structure and role of 5S RNA-protein complexes in protein biosynthesis

    R.A. Garrett;S. Douthwaite;H.F. Noller

  • Methyltransferase Erm(37) Slips on rRNA to Confer Atypical Resistance in Mycobacterium tuberculosis

    Christian Toft Madsen;Lene Jakobsen;Karolina Buriánková;Florence Doucet-Populaire

  • Isolation of temperature-sensitive mutants of 16 S rRNA in Escherichia coli.

    Kathleen Triman;Elisa Becker;Carol Dammel;John Katz

  • Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger

    Bjarne R. Poulsen;Jane Nøhr;Stephen Douthwaite;Line V. Hansen

  • YibK is the 2′-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNALeu isoacceptors

    Alfonso Benítez-Páez;Magda Villarroya;Stephen Douthwaite;Toni Gabaldón

  • YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962.

    Elzbieta Purta;Elzbieta Purta;Michelle O’Connor;Michelle O’Connor;Janusz M. Bujnicki;Janusz M. Bujnicki;Stephen Douthwaite

  • YgdE is the 2'-O-ribose methyltransferase RlmM specific for nucleotide C2498 in bacterial 23S rRNA.

    Elzbieta Purta;Elzbieta Purta;Michelle O'Connor;Michelle O'Connor;Janusz M. Bujnicki;Janusz M. Bujnicki;Stephen Douthwaite

  • YbeA is the m3Ψ methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA

    Elzbieta Purta;Katarzyna H. Kaminska;Joanna M. Kasprzak;Janusz M. Bujnicki

Frequent Co-Authors

Jesper Wengel
Jesper Wengel Aalborg University
Henri Grosjean
Henri Grosjean Centre national de la recherche scientifique, CNRS
Harry F. Noller
Harry F. Noller University of California, Santa Cruz
Alain Blanchard
Alain Blanchard University of Bordeaux
Bruno Gonzalez-Zorn
Bruno Gonzalez-Zorn Complutense University of Madrid
Harald Huber
Harald Huber University of Regensburg
Roger A. Garrett
Roger A. Garrett University of Copenhagen
Alexander S. Mankin
Alexander S. Mankin University of Illinois at Chicago
Nils-Göran Larsson
Nils-Göran Larsson Karolinska Institute

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