World's Best Scientists 2026 revealed!
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Biology and Biochemistry
Czechia
2026

D-Index & Metrics

Biology and Biochemistry

D-Index
66
Citations
37553
World Ranking
8432
National Ranking
8

Research.com Recognitions

  • 2026 - Research.com Biology and Biochemistry in Czechia Leader Award
  • 2025 - Research.com Biology and Biochemistry in Czechia Leader Award
  • 2023 - Research.com Biology and Biochemistry in Czechia Leader Award
  • 2022 - Research.com Biology and Biochemistry in Czechia Leader Award

Overview

Mikael Kubista is affiliated with the Czech Academy of Sciences in the Czech Republic and has contributed significantly to the field of Biochemistry, Genetics, and Molecular Biology, with a particular focus on Molecular Biology. Their research encompasses several subfields including Molecular Biology, Cancer Research, Neurology, Immunology, and Biomedical Engineering.

The scientist's main topics of research cover areas such as Molecular Biology Techniques and Applications, Single-cell and spatial transcriptomics, Neuroinflammation and Neurodegeneration Mechanisms, Cancer-related molecular mechanisms research, MicroRNA in disease regulation, RNA modifications and cancer, and Immune cells in cancer.

They have published frequently in several academic venues, including:

  • Frontiers in Cellular Neuroscience
  • Clinical Chemistry
  • Glia
  • bioRxiv (Cold Spring Harbor Laboratory)
  • The AAPS Journal

Mikael Kubista has collaborated extensively with multiple co-authors, with notable frequent collaborators being:

  • Lukáš Valihrach (17 collaborations)
  • Pavel Abaffy (13 collaborations)
  • Daniel Žucha (11 collaborations)
  • Miroslava Anděrová (6 collaborations)
  • Michael W. Pfaffl (5 collaborations)

Recent publications by this scientist include:

  • The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020, 2020, Clinical Chemistry
  • Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain, 2020, Cell Reports
  • Small RNA-Sequencing: Approaches and Considerations for miRNA Analysis, 2021, Diagnostics
  • Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2, 2020, Clinical Chemistry
  • Reactive astrogliosis in the era of single-cell transcriptomics, 2023, Frontiers in Cellular Neuroscience

Best Publications

  • The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

    Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

  • The real-time polymerase chain reaction

    Mikael Kubista;José Manuel Andrade;Martin Bengtsson;Amin Forootan

  • Absorption and fluorescence properties of fluorescein

    Robert Sjöback;Jan Nygren;Mikael Kubista

  • The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

    Jim F. Huggett;Carole A. Foy;Vladimir Benes;Kerry Emslie

  • DNA tetraplex formation in the control region of c-myc.

    Tomas Simonsson;Petr Pecinka;Mikael Kubista

  • Protective Role of Reactive Astrocytes in Brain Ischemia

    Lizhen Li;Andrea Lundkvist;Daniel Andersson;Ulrika Wilhelmsson

  • The interactions between the fluorescent dye thiazole orange and DNA

    Jan Nygren;Nicke Svanvik;Mikael Kubista

  • How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

    David Svec;Ales Tichopad;Vendula Novosadova;Michael W. Pfaffl

  • Properties of the Reverse Transcription Reaction in mRNA Quantification

    Anders Ståhlberg;Joakim Håkansson;Xiaojie Xian;Henrik Semb

  • Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR).

    Amin Forootan;Robert Sjöback;Jens Björkman;Björn Sjögreen

  • Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels

    Martin Bengtsson;Anders Ståhlberg;Patrik Rorsman;Mikael Kubista

  • Sequence motifs and free energies of selected natural and non-natural nucleosome positioning DNA sequences.

    A Thåström;P.T Lowary;H.R Widlund;H Cao

  • Linear dichroism spectroscopy of nucleic acids.

    Bengt Norden;Mikael Kubista;Tomas Kurucsev

  • Characterization of interaction between DNA and 4',6-diamidino-2-phenylindole by optical spectroscopy

    Mikael Kubista;Björn Åkerman;Bengt Nordén

  • Light-Up Probes: Thiazole Orange-Conjugated Peptide Nucleic Acid for Detection of Target Nucleic Acid in Homogeneous Solution

    Nicke Svanvik;Gunnar Westman;Dongyuan Wang;Mikael Kubista

  • The need for transparency and good practices in the qPCR literature

    Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

  • Comparison of Reverse Transcriptases in Gene Expression Analysis

    Anders Ståhlberg;Mikael Kubista;Michael Pfaffl

  • Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells.

    Lan-Feng Dong;Jaromira Kovarova;Martina Bajzikova;Ayenachew Bezawork-Geleta

  • Identification and Characterization of Genomic Nucleosome-positioning Sequences

    Hans R Widlund;Hui Cao;Stina Simonsson;Elisabet Magnusson

  • Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells.

    Martina Bajzikova;Martina Bajzikova;Jaromira Kovarova;Ana R. Coelho;Ana R. Coelho;Stepana Boukalova

Frequent Co-Authors

Anders Ståhlberg
Anders Ståhlberg Sahlgrenska University Hospital
Bengt Nordén
Bengt Nordén Chalmers University of Technology
Masayuki Takahashi
Masayuki Takahashi Tokyo Institute of Technology
Bo Albinsson
Bo Albinsson Chalmers University of Technology
Michael W. Pfaffl
Michael W. Pfaffl Technical University of Munich
Carl T. Wittwer
Carl T. Wittwer University of Utah
Jiri Neuzil
Jiri Neuzil Czech Academy of Sciences
Stephen A. Bustin
Stephen A. Bustin Anglia Ruskin University
Vladimir Benes
Vladimir Benes European Molecular Biology Laboratory
Jo Vandesompele
Jo Vandesompele Ghent University

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