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Biology and Biochemistry

D-Index
62
Citations
51426
World Ranking
10494
National Ranking
798

Overview

Stephen A. Bustin is affiliated with Anglia Ruskin University in the United Kingdom and has an extensive research output in the fields of biochemistry, genetics, and molecular biology as well as medicine. Their work spans a wide range of topics within these disciplines, focusing primarily on molecular biology techniques and applications, as well as infectious diseases and biomedical engineering.

Among the main topics covered in their research are:

  • Molecular Biology Techniques and Applications
  • SARS-CoV-2 detection and testing
  • Advanced biosensing and bioanalysis techniques
  • Biosensors and Analytical Detection
  • Sexual function and dysfunction studies
  • Machine Learning in Materials Science
  • Urological Disorders and Treatments

Their subfields of study highlight a concentration on:

  • Molecular Biology
  • Infectious Diseases
  • Biomedical Engineering
  • Psychiatry and Mental Health
  • Urology

Stephen A. Bustin has published extensively in numerous journals. The most frequent publication venues include:

  • International Journal of Molecular Sciences (11 publications)
  • The Journal of Sexual Medicine (5 publications)
  • Preprints.org (4 publications)
  • Clinical Chemistry (3 publications)
  • Scientific Reports (2 publications)

Recent papers published by Stephen A. Bustin and their collaborators cover topics related to quantitative PCR techniques, SARS-CoV-2 detection, and molecular medicine. Notable publications include:

  • "RT-qPCR Testing of SARS-CoV-2: A Primer," 2020, International Journal of Molecular Sciences
  • "Advances in Molecular Medicine: Unravelling Disease Complexity and Pioneering Precision Healthcare," 2023, International Journal of Molecular Sciences

Other significant papers relevant to the field of molecular detection and assay variability include works by frequent coauthors, signaling overlapping research interests:

  • "The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020," 2020, Clinical Chemistry
  • "Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance," 2021, Water Research
  • "Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2," 2020, Clinical Chemistry

Frequent coauthors associated with Stephen A. Bustin's work include:

  • Tania Nolan
  • Sara Kirvell
  • Gregory L. Shipley
  • Jim F. Huggett
  • Selim Cellek

Best Publications

  • The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

    Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

  • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.

    SA Bustin

  • Quantification of mRNA using real-time RT-PCR

    Tania Nolan;Rebecca E Hands;Stephen A Bustin

  • Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.

    S A Bustin

  • Real-time RT-PCR normalisation; strategies and considerations

    J Huggett;K Dheda;K Dheda;S Bustin;A Zumla

  • The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans

    Sharmilee Gnanapavan;Blerina Kola;Stephen A. Bustin;Damian G. Morris

  • Quantitative real-time RT-PCR – a perspective

    S A Bustin;V Benes;T Nolan;M W Pfaffl

  • Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction.

    Stephen A Bustin;Tania Nolan

  • Validation of housekeeping genes for normalizing RNA expression in real-time PCR

    Keertan Dheda;Jim F. Huggett;Stephen A. Bustin;Margaret A. Johnson

  • The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

    Jim F. Huggett;Carole A. Foy;Vladimir Benes;Kerry Emslie

  • MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

    Stephen A Bustin;Jean-François Beaulieu;Jim Huggett;Rolf Jaggi

  • The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

    K. Dheda;J.F. Huggett;J.S. Chang;L.U. Kim

  • Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies ☆

    Carmela Tricarico;Pamela Pinzani;Simonetta Bianchi;Milena Paglierani

  • Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis.

    Stephen A. Bustin;Reinhold Mueller

  • A-Z of Quantitative PCR

    Stephen A. Bustin

  • The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors.

    Márta Korbonits;Stephen A. Bustin;Masayasu Kojima;Suzanne Jordan

  • Why the need for qPCR publication guidelines?--The case for MIQE.

    Stephen A. Bustin

  • Vitamin D Receptor (VDR) mRNA and VDR Protein Levels in Relation to Vitamin D Status, Insulin Secretory Capacity, and VDR Genotype in Bangladeshi Asians

    Babatunji-William Ogunkolade;Barbara J. Boucher;Jean M. Prahl;Stephen A. Bustin

  • The need for transparency and good practices in the qPCR literature

    Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

  • Real-Time PCR

    Stephen A. Bustin

Frequent Co-Authors

Jim F. Huggett
Jim F. Huggett University College London
Jo Vandesompele
Jo Vandesompele Ghent University
Michael W. Pfaffl
Michael W. Pfaffl Technical University of Munich
Vladimir Benes
Vladimir Benes European Molecular Biology Laboratory
Carl T. Wittwer
Carl T. Wittwer University of Utah
Mikael Kubista
Mikael Kubista Czech Academy of Sciences
Jeremy A. Garson
Jeremy A. Garson University College London
Ashley B. Grossman
Ashley B. Grossman University of Oxford
Keertan Dheda
Keertan Dheda University of Cape Town
Irene M. Leigh
Irene M. Leigh University of Dundee

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