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Microbiology

D-Index
55
Citations
33634
World Ranking
3761
National Ranking
334

Overview

Jim F. Huggett is affiliated with University College London in the United Kingdom. Their research spans multiple aspects of biochemistry, genetics, and molecular biology, with significant attention to medicine. The primary areas of focus include molecular biology, infectious diseases, biomedical engineering, epidemiology, and cancer research.

The scientist has contributed extensively to topics such as molecular biology techniques and applications, SARS-CoV-2 detection and testing, biosensors and analytical detection, SARS-CoV-2 and COVID-19 research, viral gastroenteritis research and epidemiology, clinical laboratory practices and quality control, and cancer genomics and diagnostics.

Frequent collaborators in their work include Denise M. O'Sullivan, Heinz Zeichhardt, Martin Kammel, Megan H. Cleveland, and Mojca Milavec. These partnerships have resulted in numerous publications across several scientific venues.

Notable publication venues where Jim F. Huggett has published include Clinical Chemistry and Laboratory Medicine (CCLM), Clinical Chemistry, bioRxiv (Cold Spring Harbor Laboratory), SSRN Electronic Journal, and Methods.

Among recent papers contributed by Jim F. Huggett are:

  • The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 (2020) - Clinical Chemistry
  • Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study (2020) - Microbial Genomics
  • The Dangers of Using Cq to Quantify Nucleic Acid in Biological Samples: A Lesson From COVID-19 (2021) - Clinical Chemistry
  • Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2 (2020) - Clinical Chemistry
  • STROBE-metagenomics: a STROBE extension statement to guide the reporting of metagenomics studies (2020) - The Lancet Infectious Diseases

Best Publications

  • The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

    Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

  • Real-time RT-PCR normalisation; strategies and considerations

    J Huggett;K Dheda;K Dheda;S Bustin;A Zumla

  • Validation of housekeeping genes for normalizing RNA expression in real-time PCR

    Keertan Dheda;Jim F. Huggett;Stephen A. Bustin;Margaret A. Johnson

  • The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

    Jim F. Huggett;Carole A. Foy;Vladimir Benes;Kerry Emslie

  • MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

    Stephen A Bustin;Jean-François Beaulieu;Jim Huggett;Rolf Jaggi

  • The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

    K. Dheda;J.F. Huggett;J.S. Chang;L.U. Kim

  • Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs.

    Jody E. Phelan;Denise M. O’Sullivan;Diana Machado;Jorge Ramos

  • Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool

    Jim F. Huggett;Simon Cowen;Carole A. Foy

  • Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

    Alexandra S. Whale;Jim F. Huggett;Simon Cowen;Valerie Speirs

  • Lung Remodeling in Pulmonary Tuberculosis

    Keertan Dheda;Helen Booth;Jim F. Huggett;Margaret A. Johnson

  • Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification

    Alison S. Devonshire;Alexandra S. Whale;Alice Gutteridge;Gerwyn Jones

  • qPCR primer design revisited.

    Stephen A. Bustin;Jim Huggett

  • Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon

    Jim F Huggett;Tanya Novak;Jeremy A Garson;Clare Green

  • Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study

    Päivi Tissari;Alimuddin Zumla;Eveliina Tarkka;Sointu Mero

  • Fundamentals of multiplexing with digital PCR

    Alexandra S. Whale;Jim F. Huggett;Svilen Tzonev

  • Rapid and Accurate Detection of Mycobacterium tuberculosis in Sputum Samples by Cepheid Xpert MTB/RIF Assay-A Clinical Validation Study

    Andrea Rachow;Alimuddin Zumla;Norbert Heinrich;Gabriel Rojas-Ponce

  • Evaluation of Digital PCR for Absolute RNA Quantification

    Rebecca Sanders;Deborah Jane Mason;Carole A. Foy;Jim F. Huggett

  • Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus

    Gavin Nixon;Jeremy A Garson;Paul Grant;Eleni Nastouli

  • Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

    Davina Gale;Andrew R. J. Lawson;Karen Howarth;Mikidache Madi

  • Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods.

    Clare Green;Jim F Huggett;Elizabeth Talbot;Peter Mwaba

  • Utility of the antigen-specific interferon-gamma assay for the management of tuberculosis.

    Keertan Dheda;Zarir F. Udwadia;Jim F. Huggett;Margaret A. Johnson

Frequent Co-Authors

Alimuddin Zumla
Alimuddin Zumla University College London
Stephen A. Bustin
Stephen A. Bustin Anglia Ruskin University
Keertan Dheda
Keertan Dheda University of Cape Town
Graham A. W. Rook
Graham A. W. Rook University College London
Jeremy A. Garson
Jeremy A. Garson University College London
Jo Vandesompele
Jo Vandesompele Ghent University
Taane G. Clark
Taane G. Clark London School of Hygiene & Tropical Medicine
Michael W. Pfaffl
Michael W. Pfaffl Technical University of Munich
Vladimir Benes
Vladimir Benes European Molecular Biology Laboratory
Robert F. Miller
Robert F. Miller University College London

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