2026 Michael W. Pfaffl: Biology and Biochemistry Researcher – H-Index, Publications & Awards

Discipline name	D-Index	World Ranking	Current World Ranking	National Ranking	Current National Ranking	Publications	Citations
Biology and Biochemistry	66	8417	7769	615	582	288	102902

Overview

Michael W. Pfaffl is affiliated with the Technical University of Munich in Germany. Their research spans multiple disciplines within biochemistry, genetics, molecular biology, and medicine.

Their primary fields of study involve biochemistry, genetics, and molecular biology, with significant contributions in medicine. They focus on subfields such as molecular biology, cancer research, immunology, agronomy and crop science, and biomedical engineering.

Key topics in their research include:

Extracellular vesicles in disease
MicroRNA in disease regulation
Milk quality and mastitis in dairy cows
Reproductive physiology in livestock
Molecular biology techniques and applications
Circular RNAs in diseases
Histone deacetylase inhibitors research

Pfaffl has published extensively, with recent papers including:

"The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020" (2020), Clinical Chemistry
"Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target" (2022), Nature Chemical Biology
"Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo" (2023), Nature Communications
"Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2" (2020), Clinical Chemistry
"Diagnostic potential of circulating cell-free microRNAs for community-acquired pneumonia and pneumonia-related sepsis" (2020), Journal of Cellular and Molecular Medicine

Frequent coauthors working alongside Pfaffl include:

Benedikt Kirchner
Marlene Reithmair
Christian Grätz
Florian Brandes
Gustav Schelling

Pfaffl's publications are often found in specific journals and venues, notably:

Extracellular Vesicles and Circulating Nucleic Acids
Animals
Clinical Chemistry
Nature Communications
Frontiers in Immunology

Best Publications

A new mathematical model for relative quantification in real-time RT-PCR.

Michael W. Pfaffl

37240
Citations
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

17450
Citations
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Clotilde Théry;Kenneth W. Witwer;Elena Aikawa;Maria Jose Alcaraz

12011
Citations
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

Michael W. Pfaffl;Graham W. Horgan;Leo Dempfle

8516
Citations
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.

Michael W. Pfaffl;Ales Tichopad;Christian Prgomet;Tanja P. Neuvians

5343
Citations
RNA integrity and the effect on the real-time qRT-PCR performance

Simone Fleige;Michael W. Pfaffl

1859
Citations
Quantitative real-time RT-PCR – a perspective

S A Bustin;V Benes;T Nolan;M W Pfaffl

1814
Citations
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

Jan Van Deun;Pieter Mestdagh;Patrizia Agostinis;Özden Akay

1047
Citations
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

Jim F. Huggett;Carole A. Foy;Vladimir Benes;Kerry Emslie

909
Citations
Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR.

Simone Fleige;Vanessa Walf;Silvia Huch;Christian Prgomet

721
Citations
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper

Bogdan Mateescu;Emma J. K. Kowal;Bas W. M. van Balkom;Sabine Bartel

671
Citations
Standardized determination of real‐time PCR efficiency from a single reaction set‐up

Ales Tichopad;Michael Dilger;Gerhard Schwarz;Michael W. Pfaffl

629
Citations
How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

David Svec;Ales Tichopad;Vendula Novosadova;Michael W. Pfaffl

582
Citations
Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR

Michael W. Pfaffl;M. Hageleit

381
Citations
mRNA and microRNA quality control for RT-qPCR analysis

C. Becker;A. Hammerle-Fickinger;I. Riedmaier;M.W. Pfaffl

348
Citations
RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization

Tomas Hruz;Markus Wyss;Mylene Docquier;Michael W Pfaffl

332
Citations
Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications.

Jan M. Ruijter;Michael W. Pfaffl;Sheng Zhao;Andrej N. Spiess

317
Citations
The need for transparency and good practices in the qPCR literature

Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

315
Citations
Comparison of Reverse Transcriptases in Gene Expression Analysis

Anders Ståhlberg;Mikael Kubista;Michael Pfaffl

300
Citations
Normalization strategies for microRNA profiling experiments: a ‘normal’ way to a hidden layer of complexity?

Swanhild U. Meyer;Michael W. Pfaffl;Susanne E. Ulbrich

267
Citations