What is he best known for?
The fields of study he is best known for:
- Gene
- Internal medicine
- DNA
Michael W. Pfaffl mostly deals with Molecular biology, Real-time polymerase chain reaction, Gene expression, Computational biology and RNA.
His Molecular biology study combines topics in areas such as Reverse transcriptase, Polymerase chain reaction, Ligase chain reaction, Biological system and Reproducibility.
His Real-time polymerase chain reaction study integrates concerns from other disciplines, such as Reverse transcription polymerase chain reaction, Multiple displacement amplification, Amplicon and Applications of PCR.
His studies in Gene expression integrate themes in fields like Complementary DNA and Calibration curve.
His Computational biology research includes themes of Normalization, Microarray analysis techniques, microRNA, DNA sequencing and Profiling.
The concepts of his RNA study are interwoven with issues in Messenger RNA and Position paper.
His most cited work include:
- A new mathematical model for relative quantification in real-time RT-PCR. (24640 citations)
- The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments (9052 citations)
- Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR (6100 citations)
What are the main themes of his work throughout his whole career to date?
Internal medicine, Gene expression, Endocrinology, Molecular biology and microRNA are his primary areas of study.
His research investigates the connection between Internal medicine and topics such as Immune system that intersect with problems in Lactoferrin, Tumor necrosis factor alpha and Interleukin.
Michael W. Pfaffl interconnects Messenger RNA and Computational biology in the investigation of issues within Gene expression.
His Computational biology research incorporates themes from Normalization and Anabolic Agents.
His Molecular biology research is multidisciplinary, relying on both Cell culture, RNA, Reverse transcriptase, Polymerase chain reaction and Real-time polymerase chain reaction.
His research on microRNA also deals with topics like
- Biomarker and related Anabolism, Bioinformatics and Biomarker discovery,
- Cell biology together with Regulation of gene expression.
He most often published in these fields:
- Internal medicine (24.11%)
- Gene expression (21.43%)
- Endocrinology (21.43%)
What were the highlights of his more recent work (between 2018-2021)?
- microRNA (16.07%)
- Cell biology (8.93%)
- Gene expression (21.43%)
In recent papers he was focusing on the following fields of study:
Michael W. Pfaffl spends much of his time researching microRNA, Cell biology, Gene expression, Internal medicine and Extracellular vesicles.
His study in microRNA is interdisciplinary in nature, drawing from both Biomarker, Cancer research and RNA-Seq.
His research in the fields of Signal transduction overlaps with other disciplines such as MFGE8.
Michael W. Pfaffl focuses mostly in the field of Gene expression, narrowing it down to topics relating to Reverse transcriptase and, in certain cases, Computational biology.
His Internal medicine research is multidisciplinary, incorporating perspectives in Endocrinology and Mussel.
His study focuses on the intersection of Small RNA and fields such as Transcriptome with connections in the field of Molecular biology, Venous blood, Fold change, Blood drawing and Spin column-based nucleic acid purification.
Between 2018 and 2021, his most popular works were:
- Human airway epithelial extracellular vesicle miRNA signature is altered upon asthma development (27 citations)
- The Beneficial Effect of Farm Milk Consumption on Asthma, Allergies, and Infections: From Meta-Analysis of Evidence to Clinical Trial. (20 citations)
- Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research. (18 citations)
In his most recent research, the most cited papers focused on:
- Gene
- Internal medicine
- DNA
Michael W. Pfaffl mainly investigates microRNA, Cell biology, Extracellular vesicles, Reverse transcriptase and Cancer research.
His microRNA research incorporates elements of Transcriptome and Programmed cell death.
Transcriptome is the subject of his research, which falls under Gene expression.
His work on Cell to cell communication as part of general Cell biology research is often related to Basic research, thus linking different fields of science.
His Reverse transcriptase study integrates concerns from other disciplines, such as Contamination, Genome and Computational biology.
The Cancer research study combines topics in areas such as Proteome, Peripheral blood mononuclear cell, Apoptosis and Whole blood.
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