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Michael W. Pfaffl

D-Index & Metrics

Biology and Biochemistry

D-Index
66
Citations
102902
World Ranking
8417
National Ranking
615

Michael W. Pfaffl publication distribution in Biology and Biochemistry in 2026

The chart shows the distribution of publications by all Research.com ranked scientists in the field of Biology and Biochemistry in 2026. The highlighted bar marks where Michael W. Pfaffl sits on this spectrum.

47–56 publications: 8 scientists 57–66 publications: 35 scientists 67–76 publications: 106 scientists 77–86 publications: 231 scientists 87–96 publications: 414 scientists 97–106 publications: 546 scientists 107–116 publications: 704 scientists 117–126 publications: 849 scientists 127–136 publications: 980 scientists 137–146 publications: 942 scientists 147–156 publications: 969 scientists 157–166 publications: 950 scientists 167–176 publications: 951 scientists 177–186 publications: 915 scientists 187–196 publications: 787 scientists 197–206 publications: 841 scientists 207–216 publications: 735 scientists 217–226 publications: 709 scientists 227–236 publications: 651 scientists 237–246 publications: 605 scientists 247–256 publications: 510 scientists 257–266 publications: 524 scientists 267–276 publications: 434 scientists 277–286 publications: 418 scientists 287–296 publications: 350 scientists 297–306 publications: 363 scientists 307–316 publications: 315 scientists 317–326 publications: 296 scientists 327–336 publications: 261 scientists 337–346 publications: 240 scientists 347–356 publications: 219 scientists 357–366 publications: 197 scientists 367–376 publications: 154 scientists 377–386 publications: 161 scientists 387–396 publications: 155 scientists 397–406 publications: 145 scientists 407–416 publications: 124 scientists 417–426 publications: 112 scientists 427–436 publications: 132 scientists 437–446 publications: 116 scientists 447–456 publications: 99 scientists 457–466 publications: 81 scientists 467–476 publications: 91 scientists 477–486 publications: 80 scientists 487–496 publications: 80 scientists 497–506 publications: 60 scientists 507–516 publications: 36 scientists 517–526 publications: 46 scientists 527–536 publications: 54 scientists 537–546 publications: 44 scientists 547–556 publications: 43 scientists 557–566 publications: 43 scientists 567–576 publications: 42 scientists 577–586 publications: 25 scientists 587–596 publications: 34 scientists 597–606 publications: 23 scientists 607–616 publications: 33 scientists 617–626 publications: 31 scientists 627–636 publications: 27 scientists 637–646 publications: 25 scientists 647–656 publications: 28 scientists 657–666 publications: 34 scientists 667–676 publications: 18 scientists 677–686 publications: 16 scientists 687–696 publications: 10 scientists 697–706 publications: 12 scientists 707–716 publications: 21 scientists 717–726 publications: 12 scientists 727–736 publications: 12 scientists 737–746 publications: 10 scientists 747–756 publications: 7 scientists 757–766 publications: 13 scientists 767–776 publications: 15 scientists 777–786 publications: 13 scientists 787–796 publications: 9 scientists 797–806 publications: 9 scientists 807–816 publications: 7 scientists 817–826 publications: 4 scientists 827–836 publications: 9 scientists 837–846 publications: 7 scientists 847–856 publications: 3 scientists 857–866 publications: 5 scientists 867–876 publications: 5 scientists 877–886 publications: 11 scientists 887–896 publications: 3 scientists 897–906 publications: 4 scientists 907–916 publications: 7 scientists 917–926 publications: 5 scientists 927–936 publications: 6 scientists 937–946 publications: 6 scientists 947–956 publications: 3 scientists 957–966 publications: 7 scientists 967–976 publications: 2 scientists 977–986 publications: 2 scientists 987–996 publications: 1 scientists 997–1,006 publications: 5 scientists 1,007–1,016 publications: 2 scientists 1,017–1,026 publications: 2 scientists 1,027 publications: 1 scientists 1,028+ publications: 100 scientists
47 publications 1,028+

This scientist: 288 publications — 76th percentile

76% of scientists in this discipline score the same or lower.

The last bar groups every scientist with 1,028 publications or more.

Michael W. Pfaffl D-index placement in Biology and Biochemistry in 2026

The chart shows the D-index (discipline H-index) distribution of Biology and Biochemistry scientists ranked by Research.com in 2026. The highlighted bar marks where Michael W. Pfaffl sits on this spectrum.

40–41 D-Index: 80 scientists 42–43 D-Index: 183 scientists 44–45 D-Index: 317 scientists 46–47 D-Index: 504 scientists 48–49 D-Index: 718 scientists 50–51 D-Index: 900 scientists 52–53 D-Index: 1,026 scientists 54–55 D-Index: 1,150 scientists 56–57 D-Index: 1,236 scientists 58–59 D-Index: 1,253 scientists 60–61 D-Index: 1,163 scientists 62–63 D-Index: 1,131 scientists 64–65 D-Index: 1,032 scientists 66–67 D-Index: 897 scientists 68–69 D-Index: 814 scientists 70–71 D-Index: 715 scientists 72–73 D-Index: 709 scientists 74–75 D-Index: 596 scientists 76–77 D-Index: 512 scientists 78–79 D-Index: 473 scientists 80–81 D-Index: 412 scientists 82–83 D-Index: 373 scientists 84–85 D-Index: 358 scientists 86–87 D-Index: 285 scientists 88–89 D-Index: 273 scientists 90–91 D-Index: 227 scientists 92–93 D-Index: 208 scientists 94–95 D-Index: 193 scientists 96–97 D-Index: 153 scientists 98–99 D-Index: 157 scientists 100–101 D-Index: 148 scientists 102–103 D-Index: 120 scientists 104–105 D-Index: 113 scientists 106–107 D-Index: 100 scientists 108–109 D-Index: 86 scientists 110–111 D-Index: 67 scientists 112–113 D-Index: 72 scientists 114–115 D-Index: 73 scientists 116–117 D-Index: 64 scientists 118–119 D-Index: 53 scientists 120–121 D-Index: 60 scientists 122–123 D-Index: 54 scientists 124–125 D-Index: 43 scientists 126–127 D-Index: 38 scientists 128–129 D-Index: 49 scientists 130–131 D-Index: 26 scientists 132–133 D-Index: 18 scientists 134–135 D-Index: 23 scientists 136–137 D-Index: 32 scientists 138–139 D-Index: 32 scientists 140–141 D-Index: 27 scientists 142–143 D-Index: 19 scientists 144–145 D-Index: 22 scientists 146–147 D-Index: 12 scientists 148–149 D-Index: 16 scientists 150–151 D-Index: 14 scientists 152–153 D-Index: 10 scientists 154–155 D-Index: 13 scientists 156–157 D-Index: 10 scientists 158–159 D-Index: 7 scientists 160–161 D-Index: 9 scientists 162–163 D-Index: 13 scientists 164–165 D-Index: 4 scientists 166 D-Index: 4 scientists 167+ D-Index: 98 scientists
40 D-Index 167+

This scientist: 66 D-Index — 57th percentile

57% of scientists in this discipline score the same or lower.

The last bar groups every scientist with 167 D-Index or more.

Overview

Michael W. Pfaffl is affiliated with the Technical University of Munich in Germany. Their research spans multiple disciplines within biochemistry, genetics, molecular biology, and medicine.

Their primary fields of study involve biochemistry, genetics, and molecular biology, with significant contributions in medicine. They focus on subfields such as molecular biology, cancer research, immunology, agronomy and crop science, and biomedical engineering.

Key topics in their research include:

  • Extracellular vesicles in disease
  • MicroRNA in disease regulation
  • Milk quality and mastitis in dairy cows
  • Reproductive physiology in livestock
  • Molecular biology techniques and applications
  • Circular RNAs in diseases
  • Histone deacetylase inhibitors research

Pfaffl has published extensively, with recent papers including:

  • "The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020" (2020), Clinical Chemistry
  • "Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target" (2022), Nature Chemical Biology
  • "Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo" (2023), Nature Communications
  • "Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2" (2020), Clinical Chemistry
  • "Diagnostic potential of circulating cell-free microRNAs for community-acquired pneumonia and pneumonia-related sepsis" (2020), Journal of Cellular and Molecular Medicine

Frequent coauthors working alongside Pfaffl include:

  • Benedikt Kirchner
  • Marlene Reithmair
  • Christian Grätz
  • Florian Brandes
  • Gustav Schelling

Pfaffl's publications are often found in specific journals and venues, notably:

  • Extracellular Vesicles and Circulating Nucleic Acids
  • Animals
  • Clinical Chemistry
  • Nature Communications
  • Frontiers in Immunology

Best Publications

  • A new mathematical model for relative quantification in real-time RT-PCR.

    Michael W. Pfaffl

  • The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

    Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

  • Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

    Clotilde Théry;Kenneth W. Witwer;Elena Aikawa;Maria Jose Alcaraz

  • Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

    Michael W. Pfaffl;Graham W. Horgan;Leo Dempfle

  • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.

    Michael W. Pfaffl;Ales Tichopad;Christian Prgomet;Tanja P. Neuvians

  • RNA integrity and the effect on the real-time qRT-PCR performance

    Simone Fleige;Michael W. Pfaffl

  • Quantitative real-time RT-PCR – a perspective

    S A Bustin;V Benes;T Nolan;M W Pfaffl

  • EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

    Jan Van Deun;Pieter Mestdagh;Patrizia Agostinis;Özden Akay

  • The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

    Jim F. Huggett;Carole A. Foy;Vladimir Benes;Kerry Emslie

  • Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR.

    Simone Fleige;Vanessa Walf;Silvia Huch;Christian Prgomet

  • Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper

    Bogdan Mateescu;Emma J. K. Kowal;Bas W. M. van Balkom;Sabine Bartel

  • Standardized determination of real‐time PCR efficiency from a single reaction set‐up

    Ales Tichopad;Michael Dilger;Gerhard Schwarz;Michael W. Pfaffl

  • How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

    David Svec;Ales Tichopad;Vendula Novosadova;Michael W. Pfaffl

  • Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR

    Michael W. Pfaffl;M. Hageleit

  • mRNA and microRNA quality control for RT-qPCR analysis

    C. Becker;A. Hammerle-Fickinger;I. Riedmaier;M.W. Pfaffl

  • RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization

    Tomas Hruz;Markus Wyss;Mylene Docquier;Michael W Pfaffl

  • Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications.

    Jan M. Ruijter;Michael W. Pfaffl;Sheng Zhao;Andrej N. Spiess

  • The need for transparency and good practices in the qPCR literature

    Stephen A. Bustin;Vladimir Benes;Jeremy A. Garson;Jan Hellemans

  • Comparison of Reverse Transcriptases in Gene Expression Analysis

    Anders Ståhlberg;Mikael Kubista;Michael Pfaffl

  • Normalization strategies for microRNA profiling experiments: a ‘normal’ way to a hidden layer of complexity?

    Swanhild U. Meyer;Michael W. Pfaffl;Susanne E. Ulbrich

Frequent Co-Authors

Jo Vandesompele
Jo Vandesompele Ghent University
Rupert M. Bruckmaier
Rupert M. Bruckmaier University of Bern
Vladimir Benes
Vladimir Benes European Molecular Biology Laboratory
Stephen A. Bustin
Stephen A. Bustin Anglia Ruskin University
Mikael Kubista
Mikael Kubista Czech Academy of Sciences
Carl T. Wittwer
Carl T. Wittwer University of Utah
Jim F. Huggett
Jim F. Huggett University College London
Jeremy A. Garson
Jeremy A. Garson University College London
Susanne E. Ulbrich
Susanne E. Ulbrich ETH Zurich
Fred Sinowatz
Fred Sinowatz Ludwig-Maximilians-Universität München

External Links

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Andreu Palou

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Shiwen Xu

Shiwen Xu

Northeast Agricultural University

Publications: 52

Rupert M. Bruckmaier

Rupert M. Bruckmaier

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Publications: 50

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Publications: 49

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Johns Hopkins University

Publications: 48

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Chinese Academy of Fishery Sciences

Publications: 47

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Publications: 46

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Douglas R. Tocher

Shantou University

Publications: 44

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Publications: 43

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