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Molecular Biology

D-Index
48
Citations
8011
World Ranking
2689
National Ranking
15

Overview

Ichiro Hirao is affiliated with Xenolis Pte. Ltd. in Singapore. Their research primarily spans the field of Biochemistry, Genetics and Molecular Biology, with a particular focus on Molecular Biology as a subfield. The scope of their work covers diverse topics including advanced biosensing and bioanalysis techniques, RNA and protein synthesis mechanisms, DNA and nucleic acid chemistry, mosquito-borne diseases and control, bacteriophages and microbial interactions, CRISPR and genetic engineering, as well as nanopore and nanochannel transport studies.

Their recent papers include:

  • Genetic alphabet expansion technology by creating unnatural base pairs (2020, Chemical Society Reviews)
  • High-affinity five/six-letter DNA aptamers with superior specificity enabling the detection of dengue NS1 protein variants beyond the serotype identification (2021, Nucleic Acids Research)
  • Uptake mechanisms of cell-internalizing nucleic acid aptamers for applications as pharmacological agents (2021, RSC Medicinal Chemistry)
  • Genetic Code Engineering by Natural and Unnatural Base Pair Systems for the Site-Specific Incorporation of Non-Standard Amino Acids Into Proteins (2022, Frontiers in Molecular Biosciences)
  • Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers (2021, Scientific Reports)

Frequent co-authors include:

  • Michiko Kimoto
  • Hui Pen Tan
  • Ken-ichiro Matsunaga
  • Vanessa Lim
  • William Sun

Ichiro Hirao's work has been published in several recurring scientific venues such as:

  • Nature Communications
  • bioRxiv (Cold Spring Harbor Laboratory)
  • Chemical Society Reviews
  • RSC Medicinal Chemistry
  • Nucleic Acids Research

Their research contributions address multiple specialized topics:

  • Advanced biosensing and bioanalysis techniques
  • RNA and protein synthesis mechanisms
  • DNA and Nucleic Acid Chemistry
  • Mosquito-borne diseases and control
  • Bacteriophages and microbial interactions
  • CRISPR and Genetic Engineering
  • Nanopore and Nanochannel Transport Studies

Best Publications

  • Generation of high-affinity DNA aptamers using an expanded genetic alphabet.

    Michiko Kimoto;Rie Yamashige;Ken-ichiro Matsunaga;Shigeyuki Yokoyama

  • An unnatural base pair for incorporating amino acid analogs into proteins

    Ichiro Hirao;Takashi Ohtsuki;Tsuyoshi Fujiwara;Tsuneo Mitsui

  • Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.

    Kensaku Sakamoto;Akiko Hayashi;Ayako Sakamoto;Daisuke Kiga

  • An engineered Escherichia coli tyrosyl–tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

    Daisuke Kiga;Kensaku Sakamoto;Koichiro Kodama;Takanori Kigawa

  • An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA

    Ichiro Hirao;Michiko Kimoto;Tsuneo Mitsui;Tsuyoshi Fujiwara

  • Most compact hairpin-turn structure exerted by a short DNA fragment, d(GCGAAGC) in solution: an extraordinarily stable structure resistant to nucleases and heat

    Ichiro Hirao;Gota Kawai;Satoko Yoshizawa;Yoshifumi Nishimura

  • An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules

    Michiko Kimoto;Rie Kawai;Tsuneo Mitsui;Shigeyuki Yokoyama

  • Unnatural base pair systems for DNA/RNA-based biotechnology.

    Unknown

  • GNA trinucleotide loop sequences producing extraordinarily stable DNA minihairpins.

    Satoko Yoshizawa;Gota Kawai;Kimitsuna Watanabe;Kin-ichiro Miura

  • Highly specific unnatural base pair systems as a third base pair for PCR amplification

    Rie Yamashige;Michiko Kimoto;Yusuke Takezawa;Akira Sato

  • Natural versus artificial creation of base pairs in DNA: origin of nucleobases from the perspectives of unnatural base pair studies.

    Unknown

  • Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCFAAAGC)and their effect on DNA sequencing

    Ichiro Hirao;Yoshifumi Nishimura;Yon-ichi Tagawa;Kimitsuna Watanabe

  • High-Affinity DNA Aptamer Generation Targeting von Willebrand Factor A1-Domain by Genetic Alphabet Expansion for Systematic Evolution of Ligands by Exponential Enrichment Using Two Types of Libraries Composed of Five Different Bases.

    Unknown

  • An Efficient Unnatural Base Pair for PCR Amplification

    Ichiro Hirao;Tsuneo Mitsui;Michiko Kimoto;Shigeyuki Yokoyama

  • Unnatural base pairs for specific transcription

    Takashi Ohtsuki;Michiko Kimoto;Masahide Ishikawa;Tsuneo Mitsui

  • Site-specific fluorescent labeling of RNA molecules by specific transcription using unnatural base pairs.

    Rie Kawai;Michiko Kimoto;Shuji Ikeda;Tsuneo Mitsui

  • Crystal structures of a series of RNA aptamers complexed to the same protein target.

    Siân Rowsell;Nicola J. Stonehouse;Máire A. Convery;Chris J. Adams

  • The limits of specificity: an experimental analysis with RNA aptamers to MS2 coat protein variants.

    Ichiro Hirao;Marc Spingola;David Peabody;Andrew D. Ellington

  • Nuclease resistance of an extraordinarily thermostable mini-hairpin DNA fragment, d(GCGAAGC) and its application to in vitro protein synthesis.

    Satoko Yoshizawa;Takuya Ueda;Yoshiharu Ishido;Kin ichiro Miura

  • Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma.

    Unknown

Frequent Co-Authors

Kimitsuna Watanabe
Kimitsuna Watanabe National Institute of Advanced Industrial Science and Technology
Yoshifumi Nishimura
Yoshifumi Nishimura Hiroshima University
Andrew D. Ellington
Andrew D. Ellington The University of Texas at Austin
Takuya Ueda
Takuya Ueda University of Tokyo
Andreas Marx
Andreas Marx University of Konstanz
Masayuki Endo
Masayuki Endo Kyoto University
Mikako Shirouzu
Mikako Shirouzu RIKEN Center for Biosystems Dynamics Research
Kay Diederichs
Kay Diederichs University of Konstanz
Yee Sin Leo
Yee Sin Leo Nanyang Technological University

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