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Brian F. Pfleger

Brian F. Pfleger

University of Wisconsin–Madison
United States

Overview

What is he best known for?

The fields of study he is best known for:

Enzyme
Gene
Biochemistry

Brian F. Pfleger focuses on Metabolic engineering, Biochemistry, Escherichia coli, Biosynthesis and Fatty acid. The various areas that he examines in his Metabolic engineering study include CRISPR interference, Biochemical engineering, Synthetic biology, Metabolic pathway and Cell biology. Brian F. Pfleger frequently studies issues relating to Synechococcus and Biochemistry.

His Escherichia coli study combines topics from a wide range of disciplines, such as Gene expression, Computational biology and Growth medium. His Biosynthesis research incorporates elements of SOXS, Regulon, Phage shock and Unsaturated fatty acid. His Fatty acid research is multidisciplinary, incorporating elements of Biofuel and Thioesterase.

His most cited work include:

Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes (446 citations)
Nonenzymatic Sugar Production from Biomass Using Biomass-Derived γ-Valerolactone (423 citations)
A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes. (237 citations)

What are the main themes of his work throughout his whole career to date?

His primary areas of study are Biochemistry, Metabolic engineering, Escherichia coli, Gene and Fatty acid. As part of his studies on Biochemistry, Brian F. Pfleger often connects relevant subjects like Microorganism. He interconnects Fermentation, Biochemical engineering, Fatty alcohol, Flux and Biofuel in the investigation of issues within Metabolic engineering.

His research investigates the connection with Escherichia coli and areas like Strain which intersect with concerns in High-throughput screening. His studies in Gene integrate themes in fields like Synechococcus, Computational biology and Cell biology. He studied Fatty acid and Membrane that intersect with Acyl carrier protein.

He most often published in these fields:

Biochemistry (72.03%)
Metabolic engineering (40.68%)
Escherichia coli (35.59%)

What were the highlights of his more recent work (between 2019-2021)?

Biochemistry (72.03%)
Flux (9.32%)
Metabolic engineering (40.68%)

In recent papers he was focusing on the following fields of study:

Biochemistry, Flux, Metabolic engineering, Computational biology and Gene are his primary areas of study. Much of his study explores Biochemistry relationship to Strain. His Flux research is multidisciplinary, relying on both Enzyme, Natural oils and Escherichia coli.

His research in Metabolic engineering intersects with topics in Thioesterase, Yeast, Lignocellulosic biomass, 2-Heptanone and Ketone. His work deals with themes such as Biodiesel, Biochemical engineering, Oleochemical, Fatty acid and Primary, which intersect with Yeast. Brian F. Pfleger combines subjects such as Amino acid substitution, Protein engineering and Strain engineering with his study of Computational biology.

Between 2019 and 2021, his most popular works were:

Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals. (21 citations)
Metabolic engineering of β-oxidation to leverage thioesterases for production of 2-heptanone, 2-nonanone and 2-undecanone. (3 citations)
Rewiring yeast metabolism to synthesize products beyond ethanol. (2 citations)

In his most recent research, the most cited papers focused on:

Enzyme
Gene
DNA

Brian F. Pfleger spends much of his time researching Flux, Metabolic engineering, Yeast, Biodiesel and Transposable element. Flux and Ethanol are commonly linked in his work. His Metabolic engineering research includes themes of 2-Heptanone, Bioreactor, Metabolic pathway, Combinatorial chemistry and Ketone.

His Yeast study is concerned with the field of Biochemistry as a whole. His work carried out in the field of Biodiesel brings together such families of science as Biochemical engineering, Oleochemical, Fatty acid, Primary and Natural oils. Candidate gene is closely connected to Computational biology in his research, which is encompassed under the umbrella topic of Transposable element.

This overview was generated by a machine learning system which analysed the scientist’s body of work. If you have any feedback, you can contact us here.

Best Publications

Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes

Brian F Pfleger;Douglas J Pitera;Christina D Smolke;Christina D Smolke;Jay D Keasling;Jay D Keasling.
Nature Biotechnology (2006)

569 Citations

Nonenzymatic Sugar Production from Biomass Using Biomass-Derived γ-Valerolactone

Jeremy S. Luterbacher;Jacqueline M. Rand;David Martin Alonso;Jeehoon Han.
Science (2014)

563 Citations

A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes.

Rebecca M. Lennen;Drew J. Braden;Ryan M. West;James A. Dumesic.
Biotechnology and Bioengineering (2010)

257 Citations

Application of Functional Genomics to Pathway Optimization for Increased Isoprenoid Production

Lance Kizer;Douglas J. Pitera;Brian F. Pfleger;Jay D. Keasling;Jay D. Keasling.
Applied and Environmental Microbiology (2008)

223 Citations

Common principles and best practices for engineering microbiomes

Christopher E. Lawson;William R. Harcombe;Roland Hatzenpichler;Stephen R. Lindemann.
Nature Reviews Microbiology (2019)

219 Citations

Impact of synthetic biology and metabolic engineering on industrial production of fine chemicals

David Jullesson;Florian David;Brian Pfleger;Jens Nielsen.
Biotechnology Advances (2015)

208 Citations

Microbial production of fatty acid-derived fuels and chemicals

Rebecca M Lennen;Brian F Pfleger.
Current Opinion in Biotechnology (2013)

195 Citations

Engineering Escherichia coli to synthesize free fatty acids.

Rebecca M. Lennen;Brian F. Pfleger;Brian F. Pfleger.
Trends in Biotechnology (2012)

181 Citations

Modular synthase-encoding gene involved in α-olefin biosynthesis in Synechococcus sp. strain PCC 7002.

Daniel Mendez-Perez;Matthew B. Begemann;Brian F. Pfleger;Brian F. Pfleger.
Applied and Environmental Microbiology (2011)

165 Citations

Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002

Andrew L. Markley;Matthew B. Begemann;Ryan E. Clarke;Gina C. Gordon.
ACS Synthetic Biology (2015)

159 Citations

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Frequent Co-Authors

External Links

Personal Website for Brian F. Pfleger