David M. Clarke

Research.com Recognitions

• 2023 - Research.com Biology and Biochemistry in Canada Leader Award

Overview

Best Publications

• Location of high affinity Ca 2 + -binding sites within the predicted transmembrahe domain of the sarco-plasmic reticulum Ca 2+ -ATPase

  David M. Clarke;Tip W. Loo;Giuseppe Inesi;David H. MacLennan

  700
  Citations

• Membrane topology of a cysteine-less mutant of human P-glycoprotein.

  Tip W. Loo;David M. Clarke

  340
  Citations

• Recent progress in understanding the mechanism of P-glycoprotein-mediated drug efflux.

  T.W. Loo;D.M. Clarke

  293
  Citations

• Correction of Defective Protein Kinesis of Human P-glycoprotein Mutants by Substrates and Modulators

  Tip W. Loo;David M. Clarke

  284
  Citations

• Location of the Rhodamine-binding Site in the Human Multidrug Resistance P-glycoprotein

  Tip W. Loo;David M. Clarke

  266
  Citations

• Defining the drug-binding site in the human multidrug resistance P-glycoprotein using a methanethiosulfonate analog of verapamil, MTS-verapamil

  Tip W. Loo;David M. Clarke

  263
  Citations

• Determining the dimensions of the drug-binding domain of human P-glycoprotein using thiol cross-linking compounds as molecular rulers.

  Tip W. Loo;David M. Clarke

  255
  Citations

• Rapid Purification of Human P-glycoprotein Mutants Expressed Transiently in HEK 293 Cells by Nickel-Chelate Chromatography and Characterization of their Drug-stimulated ATPase Activities

  Tip W. Loo;David M. Clarke

  253
  Citations

• Simultaneous binding of two different drugs in the binding pocket of the human multidrug resistance P-glycoprotein.

  Tip W. Loo;M.Claire Bartlett;David M. Clarke

  244
  Citations

• Substrate-induced conformational changes in the transmembrane segments of human P-glycoprotein. Direct evidence for the substrate-induced fit mechanism for drug binding.

  Tip W. Loo;M. Claire Bartlett;David M. Clarke

  240
  Citations

• Reconstitution of drug-stimulated ATPase activity following co-expression of each half of human P-glycoprotein as separate polypeptides.

  T W Loo;D M Clarke

  231
  Citations

• Covalent Modification of Human P-glycoprotein Mutants Containing a Single Cysteine in Either Nucleotide-binding Fold Abolishes Drug-stimulated ATPase Activity

  Tip W. Loo;David M. Clarke

  218
  Citations

• The "LSGGQ" motif in each nucleotide-binding domain of human P-glycoprotein is adjacent to the opposing walker A sequence.

  Tip W. Loo;M. Claire Bartlett;David M. Clarke

  217
  Citations

• Functional consequences of phenylalanine mutations in the predicted transmembrane domain of P-glycoprotein.

  T W Loo;D M Clarke

  209
  Citations

• Functional consequences of alterations to polar amino acids located in the transmembrane domain of the Ca2(+)-ATPase of sarcoplasmic reticulum.

  D M Clarke;T W Loo;D H MacLennan

  190
  Citations

• Functional consequences of glutamate, aspartate, glutamine, and asparagine mutations in the stalk sector of the Ca2+-ATPase of sarcoplasmic reticulum.

  D. M. Clarke;K. Maruyama;T. W. Loo;E. Leberer

  189
  Citations

• Correctors promote maturation of cystic fibrosis transmembrane conductance regulator (CFTR)-processing mutants by binding to the protein.

  Ying Wang;Tip W. Loo;M. Claire Bartlett;David M. Clarke

  188
  Citations

• Identification of Residues within the Drug-binding Domain of the Human Multidrug Resistance P-glycoprotein by Cysteine-scanning Mutagenesis and Reaction with Dibromobimane

  Tip W. Loo;David M. Clarke

  187
  Citations

• Identification of Residues in the Drug-binding Site of Human P-glycoprotein Using a Thiol-reactive Substrate

  Tip W. Loo;David M. Clarke

  185
  Citations

• The transmembrane domains of the human multidrug resistance P-glycoprotein are sufficient to mediate drug binding and trafficking to the cell surface.

  Tip W. Loo;David M. Clarke

  185
  Citations