Tetsushi Sakuma

Overview

Best Publications

• Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9

  Hongmei Lisa Li;Naoko Fujimoto;Noriko Sasakawa;Saya Shirai

  602
  Citations

• Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9.

  Shota Nakade;Takuya Tsubota;Yuto Sakane;Satoshi Kume

  514
  Citations

• Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells.

  Kotaro Sasaki;Shihori Yokobayashi;Tomonori Nakamura;Ikuhiro Okamoto

  499
  Citations

• Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

  Tetsushi Sakuma;Ayami Nishikawa;Satoshi Kume;Kazuaki Chayama

  403
  Citations

• MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems

  Tetsushi Sakuma;Shota Nakade;Yuto Sakane;Ken-Ichi T Suzuki

  386
  Citations

• Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

  Tomomi Aida;Keiho Chiyo;Takako Usami;Harumi Ishikubo

  332
  Citations

• Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato

  Satoru Sawai;Kiyoshi Ohyama;Shuhei Yasumoto;Hikaru Seki

  298
  Citations

• Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

  Yu Hisano;Tetsushi Sakuma;Shota Nakade;Rie Ohga

  263
  Citations

• Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.

  Tetsushi Sakuma;Hiroshi Ochiai;Takehito Kaneko;Tomoji Mashimo

  257
  Citations

• Efficient TALEN construction and evaluation methods for human cell and animal applications

  Tetsushi Sakuma;Sayaka Hosoi;Knut Woltjen;Ken Ichi Suzuki

  234
  Citations

• Simple knockout by electroporation of engineered endonucleases into intact rat embryos

  Takehito Kaneko;Tetsushi Sakuma;Takashi Yamamoto;Tomoji Mashimo

  220
  Citations

• Human Induced Pluripotent Stem Cell–Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation

  Sazia Sharmin;Atsuhiro Taguchi;Yusuke Kaku;Yasuhiro Yoshimura

  212
  Citations

• Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases

  Takahito Watanabe;Hiroshi Ochiai;Tetsushi Sakuma;Hadley W. Horch

  199
  Citations

• Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing.

  Kenya Sato;Ryo Oiwa;Wakako Kumita;Rachel Henry

  173
  Citations

• EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step

  Satoshi Ninagawa;Tetsuya Okada;Yoshiki Sumitomo;Yukiko Kamiya

  171
  Citations

• The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation.

  Tatsuo Miyamoto;Kosuke Hosoba;Hiroshi Ochiai;Ekaterina Royba

  152
  Citations

• Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases.

  Satoshi Ansai;Tetsushi Sakuma;Takashi Yamamoto;Hiroyoshi Ariga

  138
  Citations

• Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes

  Chisa Shukunami;Aki Takimoto;Yuriko Nishizaki;Yuki Yoshimoto

  137
  Citations

• Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes.

  Shunsuke Tanigawa;Mazharul Islam;Sazia Sharmin;Hidekazu Naganuma

  127
  Citations

• Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells

  Hiroshi Ochiai;Takeshi Sugawara;Tetsushi Sakuma;Takashi Yamamoto

  115
  Citations