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Biology and Biochemistry

D-Index
81
Citations
54252
World Ranking
3781
National Ranking
1871

Overview

John G. Doench is affiliated with the Broad Institute in the United States. Their research spans multiple areas within biochemistry, genetics, and molecular biology, with a significant focus on medicine. The scientist's published work highlights expertise in molecular biology, oncology, immunology, infectious diseases, and genetics, reflecting a broad range of biological disciplines.

The primary research topics covered by John G. Doench include:

  • CRISPR and Genetic Engineering
  • Interferon and immune responses
  • RNA and protein synthesis mechanisms
  • CAR-T cell therapy research
  • Advanced biosensing and bioanalysis techniques
  • Protein degradation and inhibitors
  • Epigenetics and DNA methylation

The scientist has contributed extensively to the scientific literature, with frequent publications appearing in well-known venues such as:

  • bioRxiv (Cold Spring Harbor Laboratory)
  • Nature Communications
  • Blood
  • Cancer Research
  • Nature

Among the recent papers authored or co-authored by John G. Doench are:

  • "Discovering the anticancer potential of non-oncology drugs by systematic viability profiling," 2020, Nature Cancer
  • "Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis," 2020, Nature Chemical Biology
  • "Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection," 2020, Cell
  • "Control of gasdermin D oligomerization and pyroptosis by the Ragulator-Rag-mTORC1 pathway," 2021, Cell
  • "Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity," 2020, Cell Metabolism

Collaboration is a notable aspect of their research career, with frequent co-authors including:

  • David E. Root
  • Mudra Hegde
  • Peter C. DeWeirdt
  • William C. Hahn
  • Ruth E. Hanna

John G. Doench's body of work reflects substantial contributions to the understanding of genetic editing technologies, immune response mechanisms, and cellular pathways relevant to disease. Their research encompasses both foundational molecular biology and applied medical sciences, contributing to ongoing developments in cancer biology, immunology, and infectious disease research.

Best Publications

  • Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

    Ophir Shalem;Ophir Shalem;Neville E Sanjana;Neville E Sanjana;Ella Hartenian;Xi-Shun Shi

  • Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

    John G Doench;Nicolo Fusi;Meagan Sullender;Mudra Hegde

  • A Next Generation Connectivity Map: L1000 Platform and the First 1,000,000 Profiles.

    Aravind Subramanian;Rajiv Narayan;Steven M. Corsello;Steven M. Corsello;David D. Peck

  • Specificity of microRNA target selection in translational repression

    John G. Doench;Phillip A. Sharp

  • siRNAs can function as miRNAs

    John G. Doench;Christian P. Petersen;Phillip A. Sharp

  • Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

    John G Doench;Ella Hartenian;Daniel B Graham;Zuzana Tothova

  • Dependency of a therapy-resistant state of cancer cells on a lipid peroxidase pathway

    Vasanthi S. Viswanathan;Matthew J. Ryan;Harshil D. Dhruv;Shubhroz Gill

  • In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target

    Robert T. Manguso;Robert T. Manguso;Hans W. Pope;Hans W. Pope;Margaret D. Zimmer;Margaret D. Zimmer;Flavian D. Brown

  • A major chromatin regulator determines resistance of tumor cells to T cell–mediated killing

    Deng Pan;Aya Kobayashi;Aya Kobayashi;Peng Jiang;Lucas Ferrari de Andrade

  • Discovery and Characterization of Super-Enhancer-Associated Dependencies in Diffuse Large B Cell Lymphoma

    Bjoern Chapuy;Michael R. McKeown;Charles Y. Lin;Stefano Monti

  • Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

    Kendall R. Sanson;Ruth E. Hanna;Mudra Hegde;Katherine F. Donovan

  • Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade.

    Jeffrey J. Ishizuka;Jeffrey J. Ishizuka;Robert T. Manguso;Robert T. Manguso;Collins K. Cheruiyot;Collins K. Cheruiyot;Kevin Bi;Kevin Bi

  • A GPX4-dependent cancer cell state underlies the clear-cell morphology and confers sensitivity to ferroptosis.

    Yilong Zou;Yilong Zou;Michael J. Palte;Amy A. Deik;Haoxin Li;Haoxin Li

  • Discovering the anti-cancer potential of non-oncology drugs by systematic viability profiling.

    Steven M. Corsello;Steven M. Corsello;Rohith T. Nagari;Ryan D. Spangler;Jordan Rossen

  • Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection.

    Jin Wei;Mia Madel Alfajaro;Peter C. DeWeirdt;Ruth E. Hanna

  • Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis.

    Yilong Zou;Haoxin Li;Haoxin Li;Emily T. Graham;Amy A. Deik

  • Mutational processes shape the landscape of TP53 mutations in human cancer

    Andrew O. Giacomelli;Andrew O. Giacomelli;Xiaoping Yang;Robert E. Lintner;James M. McFarland

  • Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy

    Jun Wei;Lingyun Long;Wenting Zheng;Yogesh Dhungana

  • Am I ready for CRISPR? A user's guide to genetic screens.

    John G Doench

  • A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation

    Jason D. Arroyo;Jason D. Arroyo;Alexis A. Jourdain;Alexis A. Jourdain;Sarah E. Calvo;Sarah E. Calvo;Carmine A. Ballarano;Carmine A. Ballarano

Frequent Co-Authors

David E. Root
David E. Root Broad Institute
William C. Hahn
William C. Hahn Dana-Farber Cancer Institute
Todd R. Golub
Todd R. Golub Harvard University
Jesse S. Boehm
Jesse S. Boehm Broad Institute
Benjamin L. Ebert
Benjamin L. Ebert Harvard University
W. Nicholas Haining
W. Nicholas Haining Arsenal Biosciences (United States)
Constantine S. Mitsiades
Constantine S. Mitsiades Harvard University
Francisca Vazquez
Francisca Vazquez Broad Institute
Matthew Meyerson
Matthew Meyerson Harvard University
Stuart L. Schreiber
Stuart L. Schreiber Harvard University

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