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Chemistry

D-Index
60
Citations
13656
World Ranking
9647
National Ranking
548

Overview

Florian Hollfelder is a researcher affiliated with the University of Cambridge in the United Kingdom. Their work spans multiple disciplines, prominently in biochemistry, genetics, molecular biology, and engineering. The main fields of study associated with Hollfelder include Biochemistry, Genetics and Molecular Biology with 129 publications, and Engineering with 83 publications.

Their research focuses on several specialized subfields including Molecular Biology, Biomedical Engineering, Electrical and Electronic Engineering, Genetics, and Radiology, Nuclear Medicine and Imaging. This wide range of expertise supports multidisciplinary approaches in their scientific investigations.

Major topical areas in Hollfelder's work highlight innovative techniques and applications such as:

  • Innovative Microfluidic and Catalytic Techniques Innovation
  • Electrowetting and Microfluidic Technologies
  • CRISPR and Genetic Engineering
  • Enzyme Catalysis and Immobilization
  • Microfluidic and Capillary Electrophoresis Applications
  • Pluripotent Stem Cells Research
  • 3D Printing in Biomedical Research

Frequent collaborators with Hollfelder include Tomasz S. Kamiński, Timo N. Kohler, Maximilian Gantz, Joachim De Jonghe, and Marko Hyvönen, indicating consistent scholarly relationships across multiple projects.

Hollfelder's publications are distributed across various prominent venues, with a notable number of contributions to:

  • bioRxiv (Cold Spring Harbor Laboratory) - 33 publications
  • Nature Communications - 8 publications
  • Journal of the American Chemical Society - 3 publications
  • Analytical Chemistry - 3 publications
  • Lab on a Chip - 3 publications

Selected recent papers authored or co-authored by Hollfelder include:

  • "Embryo model completes gastrulation to neurulation and organogenesis," 2022, published in Nature
  • "High-throughput total RNA sequencing in single cells using VASA-seq," 2022, published in Nature Biotechnology
  • "Spatial profiling of early primate gastrulation in utero," 2022, published in Nature
  • "Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro," 2020, published in Developmental Cell
  • "Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput," 2021, published in ACS Synthetic Biology

Best Publications

  • Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology

    Ashleigh B. Theberge;Fabienne Courtois;Yolanda Schaerli;Martin Fischlechner

  • Microdroplets: A sea of applications?

    Ansgar Huebner;Sanjiv Sharma;Monpichar Srisa-Art;Florian Hollfelder

  • Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

    Ansgar Huebner;Dan Bratton;Graeme Whyte;Min Yang;Min Yang

  • Quantitative detection of protein expression in single cells using droplet microfluidics.

    A. Huebner;M. Srisa-Art;D. Holt;C. Abell

  • Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

    Yolanda Schaerli;Robert C. Wootton;Tom Robinson;Viktor Stein

  • The role of protein dynamics in the evolution of new enzyme function

    Eleanor Campbell;Miriam Kaltenbach;Galen J Correy;Paul D Carr

  • Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics.

    Pierre-Yves Colin;Balint Kintses;Fabrice Gielen;Charlotte M Miton

  • Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

    Fabrice Gielen;Raphaelle Hours;Stephane Emond;Martin Fischlechner;Martin Fischlechner

  • What makes an enzyme promiscuous

    Ann Babtie;Nobuhiko Tokuriki;Florian Hollfelder

  • Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution

    Balint Kintses;Christopher Hein;Mark F. Mohamed;Martin Fischlechner

  • Development of quantitative cell-based enzyme assays in microdroplets.

    Ansgar Huebner;Luis F. Olguin;Daniel Bratton;Graeme Whyte

  • Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays.

    Fabienne Courtois;Luis F. Olguin;Graeme Whyte;Ashleigh B. Theberge

  • One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

    Anastasia Zinchenko;Sean R. A. Devenish;Balint Kintses;Pierre-Yves Colin

  • Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays.

    Jung-uk Shim;Rohan T. Ranasinghe;Clive A. Smith;Shehu M. Ibrahim

  • Cross-talk between Histone Modifications in Response to Histone Deacetylase Inhibitors MLL4 LINKS HISTONE H3 ACETYLATION AND HISTONE H3K4 METHYLATION

    Karl P. Nightingale;Susanne Gendreizig;Darren A. White;Charlotte Bradbury

  • Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid.

    Berna Sozen;Berna Sozen;Andy L Cox;Andy L Cox;Joachim De Jonghe;Min Bao

  • Microfluidic droplets: new integrated workflows for biological experiments.

    Balint Kintses;Liisa D van Vliet;Sean R A Devenish;Florian Hollfelder

  • An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets.

    Fabienne Courtois;Luis F. Olguin;Graeme Whyte;Daniel Bratton

  • The potential of microfluidic water-in-oil droplets in experimental biology.

    Yolanda Schaerli;Florian Hollfelder

  • Off-the-shelf proteins that rival tailor-made antibodies as catalysts

    Florian Hollfelder;Anthony J. Kirby;Dan S. Tawfik

  • Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments.

    Jung-uk Shim;Luis F. Olguin;Graeme Whyte;Duncan Scott

Frequent Co-Authors

Chris Abell
Chris Abell University of Cambridge
Wilhelm T. S. Huck
Wilhelm T. S. Huck Radboud University
Anthony J. Kirby
Anthony J. Kirby University of Cambridge
Dan S. Tawfik
Dan S. Tawfik Weizmann Institute of Science
Joshua B. Edel
Joshua B. Edel Imperial College London
Austin Smith
Austin Smith University of Exeter
Erich Bornberg-Bauer
Erich Bornberg-Bauer University of Münster
Magdalena Zernicka-Goetz
Magdalena Zernicka-Goetz California Institute of Technology
Jean-Louis Reymond
Jean-Louis Reymond University of Bern

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