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Molecular Biology

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Overview

Agamemnon J. Carpousis is a researcher affiliated with the Centre national de la recherche scientifique (CNRS) in France. Their work primarily spans the fields of Biochemistry, Genetics and Molecular Biology, with a focus on Environmental Science as well. Carpousis's research covers significant subfields including Molecular Biology, Genetics, and Ecology.

The scientist's main topics of research encompass:

  • RNA and protein synthesis mechanisms
  • Bacterial Genetics and Biotechnology
  • Bacteriophages and microbial interactions
  • RNA Research and Splicing
  • RNA modifications and cancer
  • Microbial Metabolic Engineering and Bioproduction

Agamemnon J. Carpousis has authored and coauthored several scientific papers, contributing to both experimental and conceptual advances in microbiology and RNA biology. Significant publications include:

  • "Ribosomal RNA degradation induced by the bacterial RNA polymerase inhibitor rifampicin" (2021), published in RNA
  • "The association between Hfq and RNase E in long-term nitrogen-starved Escherichia coli" (2021), published in Molecular Microbiology
  • "Compartmentalization of RNA Degradosomes in Bacteria Controls Accessibility to Substrates and Ensures Concerted Degradation of mRNA to Nucleotides" (2022), published in Annual Review of Microbiology
  • "Polyribosome-Dependent Clustering of Membrane-Anchored RNA Degradosomes To Form Sites of mRNA Degradation in Escherichia coli" (2021), published in mBio
  • "The essential role of mRNA degradation in understanding and engineering E. coli metabolism" (2021), published in Biotechnology Advances

Their frequent coauthors include:

  • Lina Hamouche
  • Muriel Cocaign-Bousquet
  • Laurence Girbal
  • Leonora Poljak
  • Lydia Hadjeras

The scientist regularly publishes in venues such as:

  • bioRxiv (Cold Spring Harbor Laboratory)
  • mBio
  • RNA
  • Molecular Microbiology
  • Annual Review of Microbiology

Carpousis's work often revolves around the molecular mechanisms of RNA degradation and metabolism in bacteria, exploring how these processes regulate gene expression and affect microbial physiology. Topics like RNA degradosome compartmentalization and ribosome-associated mRNA degradation are central to their investigations. Their research also addresses bacterial responses to environmental stresses and metabolic engineering of Escherichia coli through manipulation of RNA degradation pathways.

Best Publications

  • A DEAD-box RNA helicase in the Escherichia coli RNA degradosome

    Béatrice Py;Christopher F. Higgins;Henry M. Krisch;Agamemnon J. Carpousis

  • The RNA Degradosome of Escherichia coli: An mRNA-Degrading Machine Assembled on RNase E

    Agamemnon J. Carpousis

  • Copurification of E. coli RNAase E and PNPase: Evidence for a specific association between two enzymes important in RNA processing and degradation

    Agamemnon J. Carpousis;Agamemnon J. Carpousis;Griet Van Houwe;Claude Ehretsmann;Henry M. Krisch

  • Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoter.

    Agamemnon J. Carpousis;Jay D. Gralla

  • Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome

    Nathalie F. Vanzo;Yeun Shan Li;Béatrice Py;Erwin Blum

  • mRNA degradation. A tale of poly(A) and multiprotein machines.

    Agamemnon J Carpousis;Nathalie F Vanzo;Lelia C Raynal

  • specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site

    Claude Pierre Ehretsmann;Agamemnon James Carpousis;Henry M. Krisch

  • Interaction of RNA polymerase with lacUV5 promoter DNA during mRNA initiation and elongation. Footprinting, methylation, and rifampicin-sensitivity changes accompanying transcription initiation.

    Agamemnon J. Carpousis;Jay D. Gralla

  • The RNase E of Escherichia coli is a membrane-binding protein.

    Vanessa Khemici;Leonora Poljak;Ben F. Luisi;Agamemnon J. Carpousis

  • The RNA degradosome and poly(A) polymerase of Escherichia coli are required in vivo for the degradation of small mRNA decay intermediates containing REP‐stabilizers

    Vanessa Khemici;Agamemnon J. Carpousis

  • Running rings around RNA: a superfamily of phosphate-dependent RNases.

    Martyn F. Symmons;Mark G. Williams;Ben F. Luisi;George H. Jones

  • Polyphosphate kinase is a component of the Escherichia coli RNA degradosome.

    Erwin Blum;Beatrice Py;Agamemnon J. Carpousis;Christopher F. Higgins

  • Endonucleolytic initiation of mRNA decay in Escherichia coli.

    Agamemnon J Carpousis;Ben F Luisi;Kenneth J McDowall

  • Polyadenylation promotes degradation of 3'-structured RNA by the Escherichia coli mRNA degradosome in vitro.

    Erwin Blum;Agamemnon J. Carpousis;Christopher F. Higgins;Christopher F. Higgins

  • The RNase E of Escherichia coli has at least two binding sites for DEAD‐box RNA helicases: functional replacement of RhlB by RhlE

    Vanessa Khemici;Isabelle Toesca;Leonora Poljak;Nathalie F. Vanzo

  • The RNA degradosome: life in the fast lane of adaptive molecular evolution

    Maria Jose Marcaida;Mark A. DePristo;Vidya Chandran;Agamemnon J. Carpousis

  • Function in Escherichia coli of the non-catalytic part of RNase E: role in the degradation of ribosome-free mRNA

    Anne Leroy;Nathalie F. Vanzo;Sandra Sousa;Marc Dreyfus

  • Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation.

    Lelia C. Raynal;Agamemnon J. Carpousis

  • RNA degradosomes in bacteria and chloroplasts: classification, distribution and evolution of RNase E homologs

    Soraya Aït-Bara;Agamemnon J. Carpousis

  • mRNA degradation in procaryotes.

    C P Ehretsmann;A J Carpousis;H M Krisch

Frequent Co-Authors

Ben F. Luisi
Ben F. Luisi University of Cambridge
Muriel Cocaign-Bousquet
Muriel Cocaign-Bousquet Federal University of Toulouse Midi-Pyrénées
Christopher F. Higgins
Christopher F. Higgins Hammersmith Hospital
Jay D. Gralla
Jay D. Gralla University of California, Los Angeles
Joel Rosenbloom
Joel Rosenbloom Thomas Jefferson University
Cecília M. Arraiano
Cecília M. Arraiano Universidade Nova de Lisboa
Jaana K. H. Bamford
Jaana K. H. Bamford University of Jyväskylä
Marc Dreyfus
Marc Dreyfus École Normale Supérieure
Harald Brüssow
Harald Brüssow Nestlé (Switzerland)
Jörg Vogel
Jörg Vogel University of Würzburg

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