Hiroshi Nagashima spends much of his time researching Molecular biology, Transgene, Somatic cell, Blastocyst and Embryo. Hiroshi Nagashima works mostly in the field of Molecular biology, limiting it down to topics relating to Green fluorescent protein and, in certain cases, Surveyor nuclease assay, Electroporation and Gene expression. His studies in Transgene integrate themes in fields like Cell culture, Xenotransplantation, Transplantation, Transgenesis and Antibody.
His work deals with themes such as Gene knockout, Somatic cell nuclear transfer and Genetically modified organism, which intersect with Somatic cell. As part of the same scientific family, he usually focuses on Blastocyst, concentrating on Cloning and intersecting with Cell biology and Southern blot. His Embryo study incorporates themes from Fetus, Andrology and In vitro.
The scientist’s investigation covers issues in Andrology, Embryo, Molecular biology, Blastocyst and Transgene. The concepts of his Andrology study are interwoven with issues in Embryo culture, Cryopreservation, Reproductive technology, Immunology and Anatomy. His biological study spans a wide range of topics, including Sperm and Fetus.
His research in Molecular biology intersects with topics in Xenotransplantation, Transplantation, Cloning, Gene and Somatic cell nuclear transfer. His study explores the link between Blastocyst and topics such as Human fertilization that cross with problems in Oocyte. The Cell biology study combines topics in areas such as Embryonic stem cell and Induced pluripotent stem cell.
Hiroshi Nagashima mainly investigates Transplantation, Andrology, Genetically modified organism, Genetics and Molecular biology. Hiroshi Nagashima is interested in Vitrification, which is a field of Andrology. His Vitrification research is multidisciplinary, incorporating perspectives in Embryo cryopreservation, Biomedical engineering, Embryo and Blastocyst.
His Genetically modified organism research includes elements of Transgene, Mutant, Induced pluripotent stem cell, Cell biology and Genome editing. The various areas that Hiroshi Nagashima examines in his Transgene study include Endocrinology and Internal medicine. His Molecular biology research incorporates elements of Epigenomics, Antibody, Gene and Antigen.
Hiroshi Nagashima mainly focuses on Andrology, Internal medicine, Cryopreservation, Endocrinology and Phenotype. His work carried out in the field of Andrology brings together such families of science as Resuscitation, Pancreatic islets, Islet and Transplantation. Hiroshi Nagashima has researched Cryopreservation in several fields, including Vitrification and Histology.
His Endocrinology research incorporates themes from Dwarfism and Transgene. While the research belongs to areas of Phenotype, Hiroshi Nagashima spends his time largely on the problem of Cell biology, intersecting his research to questions surrounding Complementation, Genome editing, Induced pluripotent stem cell and Gene Knockout Techniques. His Vasculogenesis study integrates concerns from other disciplines, such as Genetically modified organism, Embryo and Blastocyst.
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Cryopreservation of porcine embryos
Hiroshi Nagashima;Mark Brenton Nottle;Naomi Kashiwazaki.
Nature (1994)
Blastocyst complementation generates exogenic pancreas in vivo in apancreatic cloned pigs
Hitomi Matsunari;Hitomi Matsunari;Hiroshi Nagashima;Hiroshi Nagashima;Masahito Watanabe;Masahito Watanabe;Kazuhiro Umeyama;Kazuhiro Umeyama.
Proceedings of the National Academy of Sciences of the United States of America (2013)
Cysteamine enhances in vitro development of porcine oocytes matured and fertilized in vitro.
C G Grupen;H Nagashima;M B Nottle.
Biology of Reproduction (1995)
Production of α1,3-galactosyltransferase gene knockout pigs expressing both human decay-accelerating factor and N-acetylglucosaminyltransferase III
Yoichi Takahagi;Tatsuya Fujimura;Shuji Miyagawa;Hiroshi Nagashima.
Molecular Reproduction and Development (2005)
Remodeling of the Major Pig Xenoantigen by N-Acetylglucosaminyltransferase III in Transgenic Pig
Shuji Miyagawa;Hiroshi Murakami;Yoichi Takahagi;Rie Nakai.
Journal of Biological Chemistry (2001)
Generation of Naive-Like Porcine-Induced Pluripotent Stem Cells Capable of Contributing to Embryonic and Fetal Development
Shuh-hei Fujishiro;Kazuaki Nakano;Yoshihisa Mizukami;Takuya Azami.
Stem Cells and Development (2013)
Cryopreservation of Porcine Embryos Derived from In Vitro-Matured Oocytes
Ritsuko Esaki;Hideto Ueda;Mayuko Kurome;Kazumasa Hirakawa.
Biology of Reproduction (2004)
Production of Transgenic-clone Pigs by the Combination of ICSI-mediated Gene Transfer with Somatic Cell Nuclear Transfer
Mayuko Kurome;Hideto Ueda;Ryo Tomii;Katsutoshi Naruse.
Transgenic Research (2006)
Dystrophin-deficient pigs provide new insights into the hierarchy of physiological derangements of dystrophic muscle
Nikolai Klymiuk;Andreas Blutke;Alexander Graf;Sabine Krause.
Human Molecular Genetics (2013)
Dominant-negative mutant hepatocyte nuclear factor 1α induces diabetes in transgenic-cloned pigs
Kazuhiro Umeyama;Masahito Watanabe;Hitoshi Saito;Mayuko Kurome.
Transgenic Research (2009)
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