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Frank Buchholz

Frank Buchholz

D-Index & Metrics

Molecular Biology

D-Index
64
Citations
19586
World Ranking
1715
National Ranking
120

Overview

Frank Buchholz is affiliated with TU Dresden in Germany and focuses predominantly on research within biochemistry, genetics, and molecular biology, with notable contributions to medicine. Their work spans several subfields, including molecular biology, genetics, oncology, immunology, and hematology.

The scientist's research topics include:

  • CRISPR and genetic engineering
  • RNA and protein synthesis mechanisms
  • CAR-T cell therapy research
  • Pluripotent stem cells research
  • Immune cell function and interaction
  • Advanced biosensing and bioanalysis techniques
  • Virus-based gene therapy research

Frequent co-authors include Maciej Paszkowski-Rogacz, Duran Sürün, Lukas Theo Schmitt, Felix Lansing, and Mirko Theis.

Publication venues where Buchholz has contributed most often include:

  • bioRxiv (Cold Spring Harbor Laboratory)
  • Nature Communications
  • Cancer Research
  • Nucleic Acids Research
  • Life Science Alliance

Several recent papers illustrate Buchholz's research focus and output:

  • "Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors," 2020, Genes
  • "Antigen presentation safeguards the integrity of the hematopoietic stem cell pool," 2022, Cell Stem Cell
  • "Prediction of designer-recombinases for DNA editing with generative deep learning," 2022, Nature Communications
  • "Efficient Correction of Oncogenic KRAS and TP53 Mutations through CRISPR Base Editing," 2022, Cancer Research
  • "Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation," 2021, Lab on a Chip

Best Publications

  • A new logic for DNA engineering using recombination in Escherichia coli

    Youming Zhang;F. Buchholz;J. P. P. Muyrers;A. F. Stewart

  • High-efficiency deleter mice show that FLPe is an alternative to Cre- loxP

    C I Rodríguez;F Buchholz;J Galloway;R Sequerra

  • A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances

    Marco Y. Hein;Nina C. Hubner;Ina Poser;Juergen Cox

  • BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.

    Ina Poser;Mihail Sarov;Mihail Sarov;James R.A. Hutchins;Jean Karim Hériché

  • Minimizing the risk of reporting false positives in large-scale RNAi screens

    Christophe J Echeverri;Philip A Beachy;Buzz Baum;Michael Boutros

  • Systematic analysis of human protein complexes identifies chromosome segregation proteins.

    James R. A. Hutchins;Yusuke Toyoda;Björn Hegemann;Ina Poser

  • Improved properties of FLP recombinase evolved by cycling mutagenesis.

    Buchholz F;Angrand Po;Stewart Af

  • Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I

    Christian G Riedel;Vittorio L Katis;Vittorio L Katis;Yuki Katou;Saori Mori

  • An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division

    Ralf Kittler;Gabriele Putz;Laurence Pelletier;Ina Poser

  • Systems survey of endocytosis by multiparametric image analysis

    Claudio Collinet;Martin Stöter;Charles R. Bradshaw;Nikolay Samusik

  • Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.

    Dun Yang;Frank Buchholz;Zhongdong Huang;Andrei Goga

  • Codon-improved Cre recombinase (iCre) expression in the mouse

    D.R. Shimshek;J. Kim;M.R. Hübner;D.J. Spergel

  • Genome-scale RNAi profiling of cell division in human tissue culture cells

    Ralf Kittler;Laurence Pelletier;Laurence Pelletier;Anne Kristine Heninger;Mikolaj Slabicki

  • Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice

    Konstantinos Anastassiadis;Jun Fu;Christoph Patsch;Shengbiao Hu

  • A Genome-Scale RNAi Screen for Oct4 Modulators Defines a Role of the Paf1 Complex for Embryonic Stem Cell Identity

    Li Ding;Maciej Paszkowski-Rogacz;Anja Nitzsche;Mikolaj Michal Slabicki

  • Different Thermostabilities of FLP and Cre Recombinases: Implications for Applied Site-Specific Recombination

    F Buchholz;L Ringrose;P O Angrand;F Rossi

  • HAUS, the 8-Subunit Human Augmin Complex, Regulates Centrosome and Spindle Integrity

    Steffen Lawo;Steffen Lawo;Mikhail Bashkurov;Michael Mullin;Mariana Gomez Ferreria

  • Alteration of Cre recombinase site specificity by substrate-linked protein evolution

    Frank Buchholz;A. Francis Stewart

  • HIV-1 Proviral DNA Excision Using an Evolved Recombinase

    Indrani Sarkar;Ilona Hauber;Ilona Hauber;Joachim Hauber;Joachim Hauber;Frank Buchholz

  • The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo.

    Ekaterina Korotkevich;Ritsuya Niwayama;Aurélien Courtois;Stefanie Friese

Frequent Co-Authors

Joachim Hauber
Joachim Hauber Heinrich-Pette-Institute
Ina Poser
Ina Poser Max Planck Society
Laurence Pelletier
Laurence Pelletier Lunenfeld-Tanenbaum Research Institute
Anthony A. Hyman
Anthony A. Hyman Max Planck Institute of Molecular Cell Biology and Genetics
Matthias Mann
Matthias Mann Max Planck Institute of Biochemistry
Bianca Habermann
Bianca Habermann Aix-Marseille University
Amir Abdollahi
Amir Abdollahi National Center for Tumor Diseases
Claus Rödel
Claus Rödel Goethe University Frankfurt

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