Izumi Kumagai

Overview

Best Publications

• Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G

  Mitsunori Shiroishi;Kouhei Tsumoto;Kimie Amano;Yasuo Shirakihara

  666
  Citations

• Fucose Depletion from Human IgG1 Oligosaccharide Enhances Binding Enthalpy and Association Rate Between IgG1 and FcγRIIIa

  Akira Okazaki;Emi Shoji-Hosaka;Kazuyasu Nakamura;Masako Wakitani

  655
  Citations

• Role of Arginine in Protein Refolding, Solubilization, and Purification

  Kouhei Tsumoto;Mitsuo Umetsu;Izumi Kumagai;Daisuke Ejima

  636
  Citations

• Practical considerations in refolding proteins from inclusion bodies

  Kouhei Tsumoto;Daisuke Ejima;Izumi Kumagai;Tsutomu Arakawa

  628
  Citations

• Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d)

  Mitsunori Shiroishi;Kimiko Kuroki;Linda Rasubala;Kouhei Tsumoto

  376
  Citations

• Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment.

  Kouhei Tsumoto;Katsutoshi Shinoki;Hidemasa Kondo;Makoto Uchikawa

  288
  Citations

• Bioassisted Room‐Temperature Immobilization and Mineralization of Zinc Oxide—The Structural Ordering of ZnO Nanoparticles into a Flower‐Type Morphology

  Mitsuo Umetsu;Masamichi Mizuta;Kouhei Tsumoto;Satoshi Ohara

  264
  Citations

• How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment.

  Mitsuo Umetsu;Kouhei Tsumoto;Masaki Hara;Kumar Ashish

  261
  Citations

• Efficient Leukocyte Ig-like Receptor Signaling and Crystal Structure of Disulfide-linked HLA-G Dimer

  Mitsunori Shiroishi;Kimiko Kuroki;Toyoyuki Ose;Linda Rasubala

  260
  Citations

• Refolding of therapeutic proteins produced in Escherichia coli as inclusion bodies.

  Satoru Misawa;Izumi Kumagai

  246
  Citations

• Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.

  Kouhei Tsumoto;Mitsuo Umetsu;Izumi Kumagai;Daisuke Ejima

  212
  Citations

• Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region

  Hiroshi Ueda;Kouhei Tsumoto;Kazuishi Kubota;Eiji Suzuki

  201
  Citations

• Dissection of protein-carbohydrate interactions in mutant hen egg-white lysozyme complexes and their hydrolytic activity.

  Katsumi Maenaka;Masaaki Matsushima;Haiwei Song;Futoshi Sunada

  140
  Citations

• Highly effective recombinant format of a humanized IgG-like bispecific antibody for cancer immunotherapy with retargeting of lymphocytes to tumor cells.

  Ryutaro Asano;Yasuhiro Watanabe;Hiroko Kawaguchi;Hidesuke Fukazawa

  130
  Citations

• Chiral recognition in the binding of helicenediamine to double strand DNA: interactions between low molecular weight helical compounds and a helical polymer.

  Shinobu Honzawa;Hitoshi Okubo;Shuzo Anzai;Masahiko Yamaguchi

  127
  Citations

• Cell-free expression of two single-chain monoclonal antibodies against lysozyme: effect of domain arrangement on the expression.

  Helmut Merk;Wolfgang Stiege;Kouhei Tsumoto;Izumi Kumagai

  123
  Citations

• Effect of the extra n-terminal methionine residue on the stability and folding of recombinant alpha-lactalbumin expressed in Escherichia coli.

  Tapan K. Chaudhuri;Katsunori Horii;Takao Yoda;Munehito Arai

  123
  Citations

• Effective treatment of an orthotopic xenograft model of human glioblastoma using an EGFR-retargeted oncolytic herpes simplex virus.

  Hiroaki Uchida;Hiroaki Uchida;Marco Marzulli;Kenji Nakano;Kenji Nakano;Kenji Nakano;William F. Goins

  116
  Citations

• Thermodynamic Consequences of Mutations in Vernier Zone Residues of a Humanized Anti-human Epidermal Growth Factor Receptor Murine Antibody, 528

  Koki Makabe;Takeshi Nakanishi;Kouhei Tsumoto;Yoshikazu Tanaka

  110
  Citations

• Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical Microscopy

  Yasufumi Takahashi;Takeshi Miyamoto;Hitoshi Shiku;Ryutaro Asano

  106
  Citations