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Kouhei Tsumoto

Kouhei Tsumoto

D-Index & Metrics

Chemistry

D-Index
69
Citations
18621
World Ranking
6138
National Ranking
387

Biology and Biochemistry

D-Index
70
Citations
19170
World Ranking
6948
National Ranking
465

Overview

Kouhei Tsumoto is affiliated with the University of Tokyo in Japan and has made contributions primarily within the fields of Biochemistry, Genetics, and Molecular Biology, alongside Medicine. Their work extensively covers subfields including Molecular Biology, Radiology, Nuclear Medicine and Imaging, Immunology, Cell Biology, and Materials Chemistry.

Tsumoto's research topics are focused on Monoclonal and Polyclonal Antibodies Research, Protein purification and stability, Glycosylation and Glycoproteins Research, Protein Structure and Dynamics, Chemical Synthesis and Analysis, Click Chemistry and Applications, and RNA and protein synthesis mechanisms.

They have published numerous papers in various scientific journals. Some recent papers include:

  • "Dual-Sensitive Nanomicelles Enhancing Systemic Delivery of Therapeutically Active Antibodies Specifically into the Brain" (2020), ACS Nano
  • "Current advances in biopharmaceutical informatics: guidelines, impact and challenges in the computational developability assessment of antibody therapeutics" (2022), mAbs
  • "GPC1 specific CAR-T cells eradicate established solid tumor without adverse effects and synergize with anti-PD-1 Ab" (2020), eLife
  • "Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin" (2023), Molecular Cell
  • "Engineering Stability, Viscosity, and Immunogenicity of Antibodies by Computational Design" (2020), Journal of Pharmaceutical Sciences

Frequent co-authors working alongside Tsumoto include Satoru Nagatoishi, Makoto Nakakido, Daisuke Kuroda, José M. M. Caaveiro, and Hiroki Akiba.

Common venues where Tsumoto publishes include Scientific Reports, bioRxiv (Cold Spring Harbor Laboratory), Journal of Biological Chemistry, Biochemical and Biophysical Research Communications, and Protein Science.

Best Publications

  • Suppression of protein interactions by arginine: a proposed mechanism of the arginine effects.

    Tsutomu Arakawa;Daisuke Ejima;Kouhei Tsumoto;Noriyuki Obeyama

  • Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G

    Mitsunori Shiroishi;Kouhei Tsumoto;Kimie Amano;Yasuo Shirakihara

  • Fucose Depletion from Human IgG1 Oligosaccharide Enhances Binding Enthalpy and Association Rate Between IgG1 and FcγRIIIa

    Akira Okazaki;Emi Shoji-Hosaka;Kazuyasu Nakamura;Masako Wakitani

  • Role of Arginine in Protein Refolding, Solubilization, and Purification

    Kouhei Tsumoto;Mitsuo Umetsu;Izumi Kumagai;Daisuke Ejima

  • Practical considerations in refolding proteins from inclusion bodies

    Kouhei Tsumoto;Daisuke Ejima;Izumi Kumagai;Tsutomu Arakawa

  • The effects of arginine on refolding of aggregated proteins: not facilitate refolding, but suppress aggregation.

    Tsutomu Arakawa;Kouhei Tsumoto

  • Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d)

    Mitsunori Shiroishi;Kimiko Kuroki;Linda Rasubala;Kouhei Tsumoto

  • Biotechnology applications of amino acids in protein purification and formulations.

    T. Arakawa;K. Tsumoto;Y. Kita;B. Chang

  • Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment.

    Kouhei Tsumoto;Katsutoshi Shinoki;Hidemasa Kondo;Makoto Uchikawa

  • Bioassisted Room‐Temperature Immobilization and Mineralization of Zinc Oxide—The Structural Ordering of ZnO Nanoparticles into a Flower‐Type Morphology

    Mitsuo Umetsu;Masamichi Mizuta;Kouhei Tsumoto;Satoshi Ohara

  • How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment.

    Mitsuo Umetsu;Kouhei Tsumoto;Masaki Hara;Kumar Ashish

  • Efficient Leukocyte Ig-like Receptor Signaling and Crystal Structure of Disulfide-linked HLA-G Dimer

    Mitsunori Shiroishi;Kimiko Kuroki;Toyoyuki Ose;Linda Rasubala

  • Structural basis for self-assembly of a cytolytic pore lined by protein and lipid

    Koji Tanaka;Jose Manuel Martinez Caaveiro;Koldo Morante;Juan Manuel González-Manãs

  • Effects of acid exposure on the conformation, stability, and aggregation of monoclonal antibodies.

    Daisuke Ejima;Kouhei Tsumoto;Harumi Fukada;Ryosuke Yumioka

  • Next Generation Drying Technologies for Pharmaceutical Applications

    Robert H. Walters;Bakul Bhatnagar;Serguei Tchessalov;Ken-Ichi Izutsu

  • Elution of antibodies from a Protein-A column by aqueous arginine solutions.

    Tsutomu Arakawa;John S Philo;Kouhei Tsumoto;Ryosuke Yumioka

  • Arginine as an effective additive in gel permeation chromatography.

    Daisuke Ejima;Ryosuke Yumioka;Tsutomu Arakawa;Kouhei Tsumoto

  • Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.

    Kouhei Tsumoto;Mitsuo Umetsu;Izumi Kumagai;Daisuke Ejima

  • Small molecule pharmacological chaperones: From thermodynamic stabilization to pharmaceutical drugs.

    Tsutomu Arakawa;Daisuke Ejima;Yoshiko Kita;Kouhei Tsumoto

  • Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region

    Hiroshi Ueda;Kouhei Tsumoto;Kazuishi Kubota;Eiji Suzuki

  • Effective elution of antibodies by arginine and arginine derivatives in affinity column chromatography.

    Daisuke Ejima;Ryosuke Yumioka;Kouhei Tsumoto;Tsutomu Arakawa

Frequent Co-Authors

Izumi Kumagai
Izumi Kumagai Tokyo University of Agriculture and Technology
Yoshikazu Tanaka
Yoshikazu Tanaka Tohoku University
Takao Hamakubo
Takao Hamakubo Nippon Medical School
Teruyuki Nagamune
Teruyuki Nagamune University of Tokyo
Kentaro Shimizu
Kentaro Shimizu University of Zurich
Isao Tanaka
Isao Tanaka Hokkaido University
Min Yao
Min Yao Hokkaido University
Katsumi Maenaka
Katsumi Maenaka Hokkaido University
Makoto Kuroda
Makoto Kuroda National Institutes of Health
Tadafumi Adschiri
Tadafumi Adschiri Tohoku University

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