Seiichi Taguchi

Overview

Best Publications

• A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme.

  Seiichi Taguchi;Miwa Yamada;Ken'ichiro Matsumoto;Kenji Tajima

  389
  Citations

• Evolution of polyhydroxyalkanoate (PHA) production system by "enzyme evolution": successful case studies of directed evolution.

  Seiichi Taguchi;Yoshiharu Doi

  181
  Citations

• Enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (PHA) copolyester by in vitro evolution of Aeromonas caviae PHA synthase.

  Tomoyasu Kichise;Seiichi Taguchi;Yoshiharu Doi

  177
  Citations

• Enhanced Synthesis of Poly(3-hydroxybutyrate) in Recombinant Escherichia coli by Means of Error-Prone PCR Mutagenesis, Saturation Mutagenesis, and In Vitro Recombination of the Type II Polyhydroxyalkanoate Synthase Gene

  Kazuma Takase;Seiichi Taguchi;Yoshiharu Doi

  177
  Citations

• Molecular cloning of two (R)-specific enoyl-CoA hydratase genes from Pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis.

  Takeharu Tsuge;Toshiaki Fukui;Hiromi Matsusaki;Seiichi Taguchi

  164
  Citations

• Alteration of substrate chain-length specificity of type II synthase for polyhydroxyalkanoate biosynthesis by in vitro evolution: in vivo and in vitro enzyme assays.

  Kazuma Takase;Ken'ichiro Matsumoto;Seiichi Taguchi;Yoshiharu Doi

  156
  Citations

• PHA synthase engineering toward superbiocatalysts for custom-made biopolymers

  Christopher T. Nomura;Seiichi Taguchi

  142
  Citations

• A Repressor Protein, PhaR, Regulates Polyhydroxyalkanoate (PHA) Synthesis via Its Direct Interaction with PHA

  Akira Maehara;Seiichi Taguchi;Tatsuaki Nishiyama;Tsuneo Yamane

  137
  Citations

• An azoreductase, aerobic NADH-dependent flavoprotein discovered from Bacillus sp.: functional expression and enzymatic characterization

  Toshihiko Ooi;Takeshi Shibata;Reiko Sato;Hiroaki Ohno

  124
  Citations

• Analysis of mutational effects of a polyhydroxybutyrate (PHB) polymerase on bacterial PHB accumulation using an in vivo assay system

  Seiichi Taguchi;Akira Maehara;Kazuma Takase;Maki Nakahara

  118
  Citations

• Microbial Production of Lactate-Enriched Poly[(R)-lactate-co-(R)-3-hydroxybutyrate] with Novel Thermal Properties

  Miwa Yamada;Ken’ichiro Matsumoto;Takanori Nakai;Seiichi Taguchi

  113
  Citations

• Enzymatic and whole-cell synthesis of lactate-containing polyesters: toward the complete biological production of polylactate

  Ken’ichiro Matsumoto;Seiichi Taguchi

  110
  Citations

• Biosynthesis and characterization of polyhydroxyalkanoate containing 5-hydroxyvalerate units: Effects of 5HV units on biodegradability, cytotoxicity, mechanical and thermal properties

  Jo-Ann Chuah;Miwa Yamada;Seiichi Taguchi;Kumar Sudesh

  109
  Citations

• Coexpression of genetically engineered 3-ketoacyl-ACP synthase III (fabH) and polyhydroxyalkanoate synthase (phaC) genes leads to short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production from glucose in Escherichia coli JM109.

  Christopher T. Nomura;Kazunori Taguchi;Seiichi Taguchi;Yoshiharu Doi

  104
  Citations

• Adjustable Mutations in Lactate (LA)-Polymerizing Enzyme for the Microbial Production of LA-Based Polyesters with Tailor-Made Monomer Composition

  Miwa Yamada;Ken'ichiro Matsumoto;Kotaro Shimizu;Shu Uramoto

  99
  Citations

• Synergistic effects of Glu130Asp substitution in the type II polyhydroxyalkanoate (PHA) synthase: enhancement of PHA production and alteration of polymer molecular weight.

  Ken'ichiro Matsumoto;Kazuma Takase;Emi Aoki;Yoshiharu Doi

  96
  Citations

• Engineering of a Cold-Adapted Protease by Sequential Random Mutagenesis and a Screening System

  Seiichi Taguchi;Akiyoshi Ozaki;Haruo Momose

  95
  Citations

• Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production

  Yuyang Song;Ken’ichiro Matsumoto;Miwa Yamada;Miwa Yamada;Aoi Gohda

  93
  Citations

• In Vitro Evolution of a Polyhydroxybutyrate Synthase by Intragenic Suppression-Type Mutagenesis

  Seiichi Taguchi;Hirofumi Nakamura;Tomohiro Hiraishi;Ichiro Yamato

  84
  Citations

• Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli.

  Miwa Yamada;Ken’ichiro Matsumoto;Shu Uramoto;Ren Motohashi

  83
  Citations