2026 John W. Sedat: Biology and Biochemistry Researcher – H-Index, Publications & Awards

Discipline name	D-Index	World Ranking	Current World Ranking	National Ranking	Current National Ranking	Publications	Citations
Biology and Biochemistry	87	2801	2578	1443	1301	194	31382

Research.com Recognitions

2009 - Member of the National Academy of Sciences

Overview

John W. Sedat is affiliated with the University of California, San Francisco in the United States. Their research primarily spans the field of Biochemistry, Genetics and Molecular Biology, with a focus extending into several subfields such as Molecular Biology, Structural Biology, Biophysics, Surfaces, Coatings and Films, and Atomic and Molecular Physics, and Optics.

The scientist's work centers on a variety of main topics including:

Advanced Electron Microscopy Techniques and Applications
Genomics and Chromatin Dynamics
Advanced Fluorescence Microscopy Techniques
Electron and X-Ray Spectroscopy Techniques
Digital Holography and Microscopy
Cell Image Analysis Techniques
RNA and protein synthesis mechanisms

Among the recent papers authored or co-authored by John W. Sedat are:

"CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging" (2020, Optica)
"Three-dimensional deconvolution processing for STEM cryotomography" (2020, Proceedings of the National Academy of Sciences)
"Entropy-regularized deconvolution of cellular cryotransmission electron tomograms" (2021, Proceedings of the National Academy of Sciences)
"Microscope-Cockpit: Python-based bespoke microscopy for bio-medical science" (2021, Wellcome Open Research)
"CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging" (2020, bioRxiv (Cold Spring Harbor Laboratory))

John W. Sedat frequently publishes in several scientific venues, including:

bioRxiv (Cold Spring Harbor Laboratory)
Proceedings of the National Academy of Sciences
Wellcome Open Research
Optica
OPAL (Open@LaTrobe) (La Trobe University)

The scientist collaborates regularly with a number of co-authors who have contributed to multiple publications, such as:

Michael Elbaum
Richard M. Parton
Ian M. Dobbie
Zvi Kam
Muthuvel Arigovindan

John W. Sedat received the Member of the National Academy of Sciences award in 2009.

Best Publications

Spatial partitioning of the regulatory landscape of the X-inactivation centre

Elphège P. Nora;Bryan R. Lajoie;Edda G. Schulz;Luca Giorgetti;Luca Giorgetti;Luca Giorgetti

3349
Citations
Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination

Mats G.L. Gustafsson;Lin Shao;Peter M. Carlton;C. J. Rachel Wang

1663
Citations
Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy

Lothar Schermelleh;Peter M. Carlton;Sebastian Haase;Lin Shao

1239
Citations
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate

Haim Giloh;John W. Sedat

1223
Citations
Polarization of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis

Guy Servant;Orion D. Weiner;Paul Herzmark;Tamás Balla

1075
Citations
Fluorescence microscopy in three dimensions.

David A. Agard;Yasushi Hiraoka;Peter Shaw;John W. Sedat

925
Citations
Interphase chromosomes undergo constrained diffusional motion in living cells.

W.F Marshall;A Straight;J.F Marko;J Swedlow

769
Citations
Microtubule nucleation by γ-tubulin-containing rings in the centrosome

Michelle Moritz;Michael B. Braunfeld;John W. Sedat;Bruce Alberts

769
Citations
Early transcription and silencing of cytokine genes underlie polarization of T helper cell subsets.

Jane L. Grogan;Markus Mohrs;Brian Harmon;Dee A. Lacy

638
Citations
Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA.

Jodi Nunnari;Wallace F. Marshall;Aaron Straight;Andrew Murray

627
Citations
Three-dimensional architecture of a polytene nucleus

David A. Agard;John W. Sedat

592
Citations
I5M: 3D widefield light microscopy with better than 100 nm axial resolution.

M. G. L. Gustafsson;D. A. Agard;J. W. Sedat

585
Citations
Perturbation of Nuclear Architecture by Long-Distance Chromosome Interactions

Abby F. Dernburg;Karl W. Broman;Jennifer C. Fung;Wallace F. Marshall

511
Citations
Direct Evidence of a Role for Heterochromatin in Meiotic Chromosome Segregation

Abby F. Dernburg;John W. Sedat;R.Scott Hawley

467
Citations
Mitosis in living budding yeast: anaphase A but no metaphase plate.

Aaron F. Straight;Wallace F. Marshall;John W. Sedat;Andrew W. Murray

460
Citations
The use of a charge-coupled device for quantitative optical microscopy of biological structures

Yasushi Hiraoka;John W. Sedat;David A. Agard

413
Citations
Determination of three-dimensional imaging properties of a light microscope system. Partial confocal behavior in epifluorescence microscopy

Y. Hiraoka;J.W. Sedat;D.A. Agard

370
Citations
Telomeres Cluster De Novo before the Initiation of Synapsis: A Three-dimensional Spatial Analysis of Telomere Positions before and during Meiotic Prophase

Hank W. Bass;Wallace F. Marshall;John W. Sedat;David A. Agard

367
Citations
A Direct Approach to the Structure of Eukaryotic Chromosomes

J. Sedat;L. Manuelidis

332
Citations
UCSF tomography: an integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction.

Shawn Q. Zheng;Bettina Keszthelyi;Eric Branlund;John M. Lyle

332
Citations