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76
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Overview

Holger Puchta is affiliated with the Karlsruhe Institute of Technology in Germany. Their research primarily focuses on the intersections of biochemistry, genetics, and molecular biology, with a specific emphasis on agricultural and biological sciences. The scientist's scholarship spans several closely related subfields including molecular biology, plant science, biotechnology, genetics, and aspects of business and international management.

The body of work covers a range of topics that reflect a deep engagement with plant genetics and genetic engineering. Main research themes include:

  • CRISPR and genetic engineering
  • Chromosomal and genetic variations
  • Plant tissue culture and regeneration
  • DNA repair mechanisms
  • Plant virus research studies
  • Photosynthetic processes and mechanisms
  • Plant genetic and mutation studies

Holger Puchta has published frequently in several scientific journals. The most represented venues in their publication record are:

  • Nature Plants
  • Plant Biotechnology Journal
  • New Phytologist
  • The Plant Journal
  • The Plant Cell

Recent papers authored or co-authored by Holger Puchta include:

  • "CRISPR-Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis," 2020, Nature Plants
  • "Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering," 2020, Nature Communications
  • "ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis," 2021, Proceedings of the National Academy of Sciences
  • "CRISPR-Cas-mediated chromosome engineering for crop improvement and synthetic biology," 2021, Nature Plants
  • "Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a," 2021, Plant Biotechnology Journal

The scientist collaborates with several frequent co-authors, including:

  • Patrick Schindele
  • Andreas Houben
  • A Dorn
  • Michelle Rönspies
  • Carla Schmidt

Best Publications

  • Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.

    Friedrich Fauser;Simon Schiml;Holger Puchta

  • The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution

    Holger Puchta

  • Elevated UV-B radiation reduces genome stability in plants

    Gerhard Ries;Werner Heller;Holger Puchta;Heinrich Sandermann

  • Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination

    Holger Puchta;Bernard Dujon;Barbara Hohn

  • Capture of genomic and T‐DNA sequences during double‐strand break repair in somatic plant cells

    Siegfried Salomon;Holger Puchta

  • The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

    Simon Schiml;Friedrich Fauser;Holger Puchta

  • Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease

    Holger Puchta;Bernard Dujon;Barbara Hohn

  • Towards CRISPR/Cas crops - bringing together genomics and genome editing.

    Armin Patrick Scheben;Felix Wolter;Jacqueline Batley;Holger Puchta

  • Applying CRISPR/Cas for genome engineering in plants: the best is yet to come

    Holger Puchta

  • Synthetic nucleases for genome engineering in plants: prospects for a bright future.

    Holger Puchta;Friedrich Fauser

  • Highly efficient heritable plant genome engineering using Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus.

    Jeannette Steinert;Simon Schiml;Friedrich Fauser;Holger Puchta

  • In planta gene targeting

    Friedrich Fauser;Nadine Roth;Michael Pacher;Gabriele Ilg

  • Species‐specific double‐strand break repair and genome evolution in plants

    Angela Kirik;Siegfried Salomon;Holger Puchta

  • Elimination of selection markers from transgenic plants.

    Barbara Hohn;Avraham A Levy;Holger Puchta

  • Gene targeting in plants: 25 years later.

    Holger Puchta;Friedrich Fauser

  • Intrachromosomal homologous recombination in whole plants.

    Peter Swoboda;Susannah Gal;Barbara Hohn;Holger Puchta

  • Gene replacement by homologous recombination in plants.

    Holger Puchta

  • Two closely related RecQ helicases have antagonistic roles in homologous recombination and DNA repair in Arabidopsis thaliana

    Frank Hartung;Stefanie Suer;Holger Puchta

  • The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in Arabidopsis

    Frank Hartung;Rebecca Wurz-Wildersinn;Jörg Fuchs;Ingo Schubert

  • Efficient Repair of Genomic Double-Strand Breaks by Homologous Recombination between Directly Repeated Sequences in the Plant Genome

    Ralph Siebert;Holger Puchta

Frequent Co-Authors

Barbara Hohn
Barbara Hohn Friedrich Miescher Institute
Andreas Houben
Andreas Houben Leibniz Association
Ingo Schubert
Ingo Schubert Leibniz Association
Twan Rutten
Twan Rutten Institute of Plant Genetics and Crop Plant Research
Jakob Troppmair
Jakob Troppmair Innsbruck Medical University
Andrew C.B. Cato
Andrew C.B. Cato Karlsruhe Institute of Technology
Scott B. Ficarro
Scott B. Ficarro Harvard University
Jörg Fuchs
Jörg Fuchs Leibniz Association
Uwe Strähle
Uwe Strähle Karlsruhe Institute of Technology
Bernard Dujon
Bernard Dujon Institut Pasteur

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