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Protein Expression and Purification
H-index 13

Protein Expression and Purification

Ranking & Metrics

Discipline name Position Best Scientists Publications D-Index
Molecular Biology 161 11 13 4
Biology and Biochemistry 586 66 69 9
Chemistry 723 59 57 9

Additional Metrics

Number of Best Scientists*: 155
Documents by Best Scientists*: 142
Top 100 Ranked Scientists*: 5
SCIMAGO H-index: 99
SCIMAGO SJR: 0.388
Impact Factor: 1.2

Overview

Top Research Topics at Protein Expression and Purification?

Protein Expression and Purification was organized to reinforce research efforts on Biochemistry, Molecular biology, Recombinant DNA, Escherichia coli and Enzyme. Protein Expression and Purification connects research in Biochemistry with the related topic of Chromatography. Topics in Chromatography were tackled in line with various other fields like Size-exclusion chromatography, Polyacrylamide gel electrophoresis and Yield (chemistry).

Issues in Molecular biology were discussed, taking into consideration concepts from other disciplines like Amino acid, Cell culture, Expression vector and Complementary DNA, Gene. Protein Expression and Purification addresses concerns in Recombinant DNA which are intertwined with other disciplines, such as Molecular mass, Protease, Antibody and Peptide. While work presented in Protein Expression and Purification provided substantial information on Escherichia coli, it also covered topics in Plasmid, Gene expression, Heterologous expression and Microbiology.

The work on Enzyme presented in the journal focuses on Enzyme assay in particular. Research on Affinity chromatography addressed in Protein Expression and Purification frequently intersections with the field of FLAG-tag. Pichia pastoris study tackled is connected to the field of Yeast.

  • Biochemistry (69.47%)
  • Molecular biology (39.79%)
  • Recombinant DNA (39.09%)

What are the most cited papers published in the journal?

  • Protein production by auto-induction in high-density shaking cultures (4340 citations)
  • Immobilized metal ion affinity chromatography (600 citations)
  • Overcoming Expression and Purification Problems of RhoGDI Using a Family of “Parallel” Expression Vectors☆ (570 citations)

Research areas of the most cited articles at Protein Expression and Purification:

The most cited papers aim to foster the development of research in Biochemistry, Molecular biology, Recombinant DNA, Escherichia coli and Chromatography. The most cited articles facilitate discussions on Molecular biology that incorporate concepts from other fields like Cell culture and Complementary DNA, Plasmid, Peptide sequence, Gene. The published articles focus on Escherichia coli but the discussions also offer insight into other areas such as Amino acid, Peptide and Microbiology.

What topics the last edition of the journal is best known for?

  • Enzyme
  • Gene
  • Amino acid

The previous edition focused in particular on these issues:

The journal generally zeroes in on subjects such as Biochemistry, Recombinant DNA, Fusion protein, Escherichia coli and Enzyme. Protein purification, Maltose-binding protein, DNA, Cloning and Cytidine are all topics related to Biochemistry research discussed. The research on Recombinant DNA featured in the journal combines topics in other fields like RNase P, Affinity chromatography, Protease and Homologous recombination.

The research on Affinity chromatography tackled can also make contributions to studies in the areas of In vitro, RNA, Nucleotide, Immunogenicity and Urate oxidase. While Protein Expression and Purification focused on Fusion protein, it was also able to explore topics like C-terminus and Mutant. In addition to Enzyme research, it aims to explore topics under Periplasmic space, Protein A, Protein-fragment complementation assay, Protein tag and Protein G.

The most cited articles from the last journal are:

  • Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies. (0 citations)
  • Optimizing purification of the peripheral membrane protein FAM92A1 fused to a modified spidroin tag. (0 citations)
  • Enhanced expression and purification of nucleotide-specific ribonucleases MC1 and Cusativin. (0 citations)

Papers citation over time

A key indicator for each journal is its effectiveness in reaching other researchers with the papers published at that venue.

The chart below presents the interquartile range (first quartile 25%, median 50% and third quartile 75%) of the number of citations of articles over time.

The top authors publishing in Protein Expression and Purification (based on the number of publications) are:

  • Tsutomu Arakawa (23 papers) absent at the last edition,
  • Richard R. Burgess (23 papers) absent at the last edition,
  • Brian G. Fox (17 papers) absent at the last edition,
  • Moon-Sik Yang (14 papers) absent at the last edition,
  • Józef Kur (14 papers) absent at the last edition.

The overall trend for top authors publishing in this journal is outlined below. The chart shows the number of publications at each edition of the journal for top authors.

Only papers with recognized affiliations are considered

The top affiliations publishing in Protein Expression and Purification (based on the number of publications) are:

  • Chinese Academy of Sciences (101 papers) absent at the last edition,
  • National Institutes of Health (86 papers) absent at the last edition,
  • Centre national de la recherche scientifique (73 papers) absent at the last edition,
  • University of Wisconsin-Madison (71 papers) absent at the last edition,
  • Jilin University (43 papers) absent at the last edition.

The overall trend for top affiliations publishing in this journal is outlined below. The chart shows the number of publications at each edition of the journal for top affiliations.

Publication chance based on affiliation

The publication chance index shows the ratio of articles published by the best research institutions in the journal edition to all articles published within that journal. The best research institutions were selected based on the largest number of articles published during all editions of the journal.

The chart below presents the percentage ratio of articles from top institutions (based on their ranking of total papers).Top affiliations were grouped by their rank into the following tiers: top 1-10, top 11-20, top 21-50, and top 51+. Only articles with a recognized affiliation are considered.

During the most recent 2022 edition, 9.09% of publications had an unrecognized affiliation. Out of the publications with recognized affiliations, 0.00% were posted by at least one author from the top 10 institutions publishing in the journal. Another 0.00% included authors affiliated with research institutions from the top 11-20 affiliations. Institutions from the 21-50 range included 20.00% of all publications and 80.00% were from other institutions.

Returning Authors Index

A very common phenomenon observed among researchers publishing scientific articles is the intentional selection of journals they have already attended in the past. In particular, it is worth analyzing the case when the authors participate in the same journal from year to year.

The Returning Authors Index presented below illustrates the ratio of authors who participated in both a given as well as the previous edition of the journal in relation to all participants in a given year.

Returning Institution Index

The graph below shows the Returning Institution Index, illustrating the ratio of institutions that participated in both a given and the previous edition of the conference in relation to all affiliations present in a given year.

The experience to innovation index

Our experience to innovation index was created to show a cross-section of the experience level of authors publishing in a journal. The index includes the authors publishing at the last edition of a journal, grouped by total number of publications throughout their academic career (P) and the total number of citations of these publications ever received (C).

The group intervals were selected empirically to best show the diversity of the authors' experiences, their labels were selected as a convenience, not as judgment. The authors were divided into the following groups:

  • Novice - P < 5 or C < 25 (the number of publications less than 5 or the number of citations less than 25),
  • Competent - P < 10 or C < 100 (the number of publications less than 10 or the number of citations less than 100),
  • Experienced - P < 25 or C < 625 (the number of publications less than 25 or the number of citations less than 625),
  • Master - P < 50 or C < 2500 (the number of publications less than 50 or the number of citations less than 2500),
  • Star - P ≥ 50 and C ≥ 2500 (both the number of publications greater than 50 and the number of citations greater than 2500).

The chart below illustrates experience levels of first authors in cases of publications with multiple authors.

Top Publications

  • Expression and purification of recombinant G protein-coupled receptors: A review.

    Daniel N. Wiseman;Abigail Otchere;Jaimin H. Patel;Romez Uddin

    (2020)
    54 Citations
  • Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate

    Wen-Hsiang Chen;Jeroen Pollet;Ulrich Strych;Jungsoon Lee

    (2022)
    41 Citations
  • A bacterial endo-β-1,4-glucuronan lyase, CUL-I from Brevundimonas sp. SH203, belonging to a novel polysaccharide lyase family

    Masako Kikuchi;Naotake Konno;Tomohiro Suzuki;Yuta Fujii

    (2020)
    19 Citations
  • Large scale production of synthetic spider silk proteins in Escherichia coli.

    Gargi Bhattacharyya;Paula Oliveira;Sreevidhya T. Krishnaji;Dong Chen

    (2021)
    18 Citations
  • Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles.

    (2022)
    16 Citations
  • Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide

    Ryo Sato;Kosuke Minamihata;Ryutaro Ariyoshi;Hiromasa Taniguchi

    (2020)
    15 Citations
  • Expression and characterization of L-arabinose isomerase from Geobacillus stearothermophilus for improved activity under acidic condition.

    (2020)
    15 Citations

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